Detailed information NTG0063974

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P795_RS13290
• Genome accession: NC_023028
• Coordinates: 2873991..2875109 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347040.1
• Uniprot ID: A0A809JLP7
• Organism: Acinetobacter baumannii ZW85-1
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2868991..2880109

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P795_RS13280 (P795_13275)	-	2872107..2872799 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
P795_RS13285 (P795_13280)	pilT	2872926..2873963 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
P795_RS13290 (P795_13285)	pilU	2873991..2875109 (+)	1119	WP_000347040.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
P795_RS13295 (P795_13290)	fur	2875179..2875616 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
P795_RS13300 (P795_13295)	-	2875728..2876126 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
P795_RS13305 (P795_13300)	-	2876181..2876504 (-)	324	WP_000442589.1	RnfH family protein	-
P795_RS13310 (P795_13305)	-	2876504..2877571 (-)	1068	WP_004748097.1	hypothetical protein	-
P795_RS13315 (P795_13310)	-	2877724..2878179 (-)	456	WP_000782976.1	bacteriohemerythrin	-
P795_RS13320 (P795_13315)	-	2878432..2879280 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41896.10 Da
• Isoelectric Point: 6.6406

>NTDB_id=63974 P795_RS13290 WP_000347040.1 2873991..2875109(+) (pilU) [Acinetobacter baumannii ZW85-1]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=63974 P795_RS13290 WP_000347040.1 2873991..2875109(+) (pilU) [Acinetobacter baumannii ZW85-1]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGACAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACATTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A809JLP7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392