Detailed information NTG0639549

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: LRP86_RS13205
• Genome accession: NZ_CP089973
• Coordinates: 2900631..2901695 (+)
• Length: 354 a.a.
• NCBI ID: WP_003196973.1
• Uniprot ID: A0A0Q7AAJ5
• Organism: Pseudomonas brassicacearum strain WCS365
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2895631..2906695

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRP86_RS13195 (LRP86_02629)	-	2896351..2897619 (+)	1269	WP_003196970.1	malic enzyme-like NAD(P)-binding protein	-
LRP86_RS13200 (LRP86_02630)	-	2897978..2900422 (-)	2445	WP_371916716.1	penicillin-binding protein 1A	-
LRP86_RS13205 (LRP86_02631)	comM	2900631..2901695 (+)	1065	WP_003196973.1	pilus assembly protein PilM	Machinery gene
LRP86_RS13210 (LRP86_02632)	-	2901695..2902261 (+)	567	WP_003196975.1	PilN domain-containing protein	-
LRP86_RS13215 (LRP86_02633)	pilO	2902258..2902881 (+)	624	WP_003196977.1	type 4a pilus biogenesis protein PilO	-
LRP86_RS13220 (LRP86_02634)	-	2902878..2903405 (+)	528	WP_003196979.1	pilus assembly protein PilP	-
LRP86_RS13225 (LRP86_02635)	pilQ	2903419..2905509 (+)	2091	WP_003196981.1	type IV pilus secretin PilQ	Machinery gene
LRP86_RS13230 (LRP86_02636)	aroK	2905514..2906032 (+)	519	WP_003196983.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38084.64 Da
• Isoelectric Point: 4.9070

>NTDB_id=639549 LRP86_RS13205 WP_003196973.1 2900631..2901695(+) (comM) [Pseudomonas brassicacearum strain WCS365]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVVEKNIAELEGVGQALSRLLAKAKTPLRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVMGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGQAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALAGDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=639549 LRP86_RS13205 WP_003196973.1 2900631..2901695(+) (comM) [Pseudomonas brassicacearum strain WCS365]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTGGAGTT
GAGCCGCCAGGGCGATCGATACCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCAGCCAACGCTGTGGTCGAGAAGAACA
TCGCCGAGCTCGAAGGGGTGGGGCAGGCGTTGTCGCGCCTGTTGGCCAAGGCCAAGACGCCCTTGCGTAGCGTGGCGGTG
GCGGTGGCGGGTTCAGCGGTGATCACCAAGACCATCGAGATGGACGCCGGCCTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATCCCCTATCCCCTGGATGAGGTGGCCATCGATTTCGAAGTGATGGGTGTTTCGCCAC
GCAGTGCCGAGCGCGTCGAAGTGCTGCTGGCGGCGTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCATTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCTTATGCGCTGGAGCGTGCTTTTGGCCTGCTCGCCACGCAACTGGC
TGCCTCCCAGGAACGGCTGACTGTGGCGGTCGTCGATATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGCCGCCAACTGACCGAGGAGATCCAGCGCCGCTATGGCCTGACACCC
GAGCAGGCTGGCCAGGCAAAGAAGCAGGGTGGCCTGCCGGACGACTATCTCAGCGAAGTGCTGCAACCTTTTCGCGAGGC
TCTGGTACAGCAGGTCTCGCGGTCCTTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGTGCGGTGGACCACATTCTGCTGG
CCGGGGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGCGCCCTGGCCGGTGATGCGCCGGCACTGATGATTGCCTGTGG
GCTGGCCCTCAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0Q7AAJ5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Acinetobacter nosocomialis M2	55.932	100	0.559
pilM	Acinetobacter baumannii D1279779	55.932	100	0.559
comM	Acinetobacter baylyi ADP1	54.802	100	0.548
pilM	Legionella pneumophila strain ERS1305867	47.458	100	0.475