Detailed information NTG0638469

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LRP95_RS01980
• Genome accession: NZ_CP089745
• Coordinates: 426750..427898 (+)
• Length: 382 a.a.
• NCBI ID: WP_232683443.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain Pa608
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 421750..432898

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRP95_RS01955	-	422901..423197 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
LRP95_RS01960	-	423194..423787 (-)	594	WP_003084544.1	YggT family protein	-
LRP95_RS01965	proC	423798..424619 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LRP95_RS01970	-	424631..425323 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
LRP95_RS01975	pilT	425538..426572 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LRP95_RS01980	pilU	426750..427898 (+)	1149	WP_232683443.1	type IV pilus ATPase PilU	Machinery gene
LRP95_RS01985	czcD	427906..428805 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LRP95_RS01990	-	428959..429363 (+)	405	WP_003084560.1	NINE protein	-
LRP95_RS01995	-	429606..430979 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
LRP95_RS02000	-	430976..432160 (+)	1185	WP_014602386.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42562.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=638469 LRP95_RS01980 WP_232683443.1 426750..427898(+) (pilU) [Pseudomonas aeruginosa strain Pa608]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPTGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=638469 LRP95_RS01980 WP_232683443.1 426750..427898(+) (pilU) [Pseudomonas aeruginosa strain Pa608]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTACCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382