Detailed information NTG0635950

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LSG40_RS02380
• Genome accession: NZ_CP089236
• Coordinates: 489964..491112 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain JNQH-PA027
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 484964..496112

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSG40_RS02355 (LSG40_02355)	-	486115..486411 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
LSG40_RS02360 (LSG40_02360)	-	486408..487001 (-)	594	WP_003084544.1	YggT family protein	-
LSG40_RS02365 (LSG40_02365)	proC	487012..487833 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LSG40_RS02370 (LSG40_02370)	-	487845..488537 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
LSG40_RS02375 (LSG40_02375)	pilT	488752..489786 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LSG40_RS02380 (LSG40_02380)	pilU	489964..491112 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
LSG40_RS02385 (LSG40_02385)	czcD	491120..492019 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LSG40_RS02390 (LSG40_02390)	-	492173..492577 (+)	405	WP_003084560.1	NINE protein	-
LSG40_RS02395 (LSG40_02395)	-	492820..494193 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
LSG40_RS02400 (LSG40_02400)	-	494190..495374 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=635950 LSG40_RS02380 WP_003100959.1 489964..491112(+) (pilU) [Pseudomonas aeruginosa strain JNQH-PA027]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=635950 LSG40_RS02380 WP_003100959.1 489964..491112(+) (pilU) [Pseudomonas aeruginosa strain JNQH-PA027]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382