Detailed information NTG0635894

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA14_RS02155
• Genome accession: NZ_CP106742
• Coordinates: 472920..474068 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PALA14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 467920..479068

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA14_RS02130	-	469071..469367 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PALA14_RS02135 (PALA14_00424)	-	469364..469957 (-)	594	WP_003084544.1	YggT family protein	-
PALA14_RS02140 (PALA14_00425)	proC	469968..470789 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PALA14_RS02145 (PALA14_00426)	-	470801..471493 (-)	693	WP_023464234.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA14_RS02150 (PALA14_00427)	pilT	471708..472742 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA14_RS02155 (PALA14_00428)	pilU	472920..474068 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PALA14_RS02160 (PALA14_00429)	czcD	474076..474975 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PALA14_RS02165 (PALA14_00430)	-	475129..475533 (+)	405	WP_003084560.1	NINE protein	-
PALA14_RS02170 (PALA14_00431)	-	475776..477149 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PALA14_RS02175 (PALA14_00432)	-	477146..478330 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=635894 PALA14_RS02155 WP_003100959.1 472920..474068(+) (pilU) [Pseudomonas aeruginosa strain PALA14]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=635894 PALA14_RS02155 WP_003100959.1 472920..474068(+) (pilU) [Pseudomonas aeruginosa strain PALA14]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382