Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LTQ02_RS12370 Genome accession   NZ_CP089205
Coordinates   2722658..2723287 (-) Length   209 a.a.
NCBI ID   WP_004729667.1    Uniprot ID   -
Organism   Vibrio splendidus strain 4_C08b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2717658..2728287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTQ02_RS12350 (LTQ02_12350) aroB 2718590..2719678 (-) 1089 WP_004729663.1 3-dehydroquinate synthase -
  LTQ02_RS12355 (LTQ02_12355) aroK 2719704..2720222 (-) 519 WP_004729664.1 shikimate kinase AroK -
  LTQ02_RS12360 (LTQ02_12360) pilQ 2720399..2722117 (-) 1719 WP_243585238.1 type IV pilus secretin PilQ family protein Machinery gene
  LTQ02_RS12365 (LTQ02_12365) pilP 2722153..2722671 (-) 519 WP_004729666.1 pilus assembly protein PilP Machinery gene
  LTQ02_RS12370 (LTQ02_12370) pilO 2722658..2723287 (-) 630 WP_004729667.1 type 4a pilus biogenesis protein PilO Machinery gene
  LTQ02_RS12375 (LTQ02_12375) pilN 2723280..2723879 (-) 600 WP_102488973.1 PilN domain-containing protein Machinery gene
  LTQ02_RS12380 (LTQ02_12380) pilM 2723863..2724888 (-) 1026 WP_243578739.1 type IV pilus assembly protein PilM Machinery gene
  LTQ02_RS12385 (LTQ02_12385) - 2725040..2727602 (+) 2563 Protein_2399 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 24294.86 Da        Isoelectric Point: 4.6751

>NTDB_id=635759 LTQ02_RS12370 WP_004729667.1 2722658..2723287(-) (pilO) [Vibrio splendidus strain 4_C08b]
MANLQNKISWQNRMSLQDLDVDEITEWPLLPQLLVILVLIVLIQGVGTWLYVLPLDDELQQMKQQEQTLKATLRIKANKV
AALPKLQSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNKLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGD
FSAAIAKLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDETQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=635759 LTQ02_RS12370 WP_004729667.1 2722658..2723287(-) (pilO) [Vibrio splendidus strain 4_C08b]
ATGGCTAACCTTCAAAATAAGATTAGTTGGCAAAACAGGATGAGTTTGCAAGATCTCGATGTTGATGAGATCACCGAATG
GCCATTGTTGCCTCAGCTTCTCGTGATTCTGGTGTTGATAGTGTTAATCCAAGGTGTTGGCACTTGGCTTTATGTGCTGC
CGCTCGACGATGAACTGCAACAGATGAAGCAACAGGAGCAGACCTTAAAAGCGACCTTGAGAATAAAGGCCAATAAGGTT
GCGGCCTTACCTAAGCTTCAGAGCCAGTTGGATGAGCTAACTAGCCGTTACGACTATCTATTGGAGCAATTGCCCGTTCA
AAAAGAGCTCGCCAGTATGTTGGCCTCTGTGAACGAGCTTGGATTGGATAATAAGCTGACATTTACGCGAATTGACTGGG
GGCAGAAGCAGAACAAAGAGTTTCTCTATCGTTTACCGCTCAACATCGAGCTGACGGGTGACTACCACGAGATTGGTGAT
TTCTCTGCGGCCATTGCTAAGCTGCCACGCATCATCAGCTTTGATGACGTGAATTGGCAGCGAGTCAGTCAAGAAAGTAG
CACGCTGCACTTCAGGGTTCGAGCTTATACCTACCAATTTAAATCGGAGGTCAATGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(59-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

76.166

92.344

0.703
  pilO Vibrio campbellii strain DS40M4

73.514

88.517

0.651