Detailed information NTG0635716

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: LTQ54_RS11450
• Genome accession: NZ_CP089203
• Coordinates: 2461872..2463557 (-)
• Length: 561 a.a.
• NCBI ID: WP_243578788.1
• Uniprot ID: -
• Organism: Vibrio splendidus strain 2_C04b
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2455939..2473350	2461872..2463557	within	0

Gene organization within MGE regions

Location: 2455939..2473350

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LTQ54_RS11410 (LTQ54_11410)	rimM	2455939..2456493 (+)	555	WP_004735510.1	ribosome maturation factor RimM	-
LTQ54_RS11415 (LTQ54_11415)	trmD	2456520..2457260 (+)	741	WP_004735512.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
LTQ54_RS11420 (LTQ54_11420)	rplS	2457320..2457673 (+)	354	WP_004735514.1	50S ribosomal protein L19	-
LTQ54_RS11425 (LTQ54_11425)	yacG	2458028..2458225 (-)	198	WP_004735515.1	DNA gyrase inhibitor YacG	-
LTQ54_RS11430 (LTQ54_11430)	zapD	2458307..2459047 (-)	741	WP_017087400.1	cell division protein ZapD	-
LTQ54_RS11435 (LTQ54_11435)	coaE	2459074..2459688 (-)	615	WP_017088713.1	dephospho-CoA kinase	-
LTQ54_RS11440 (LTQ54_11440)	pilD	2459691..2460560 (-)	870	WP_004735439.1	A24 family peptidase	Machinery gene
LTQ54_RS11445 (LTQ54_11445)	pilC	2460628..2461857 (-)	1230	WP_243578787.1	type II secretion system F family protein	Machinery gene
LTQ54_RS11450 (LTQ54_11450)	pilB	2461872..2463557 (-)	1686	WP_243578788.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LTQ54_RS11455 (LTQ54_11455)	pilA	2463557..2463985 (-)	429	WP_243578789.1	pilin	Machinery gene
LTQ54_RS11460 (LTQ54_11460)	nadC	2464241..2465131 (-)	891	WP_017079961.1	carboxylating nicotinate-nucleotide diphosphorylase	-
LTQ54_RS11465 (LTQ54_11465)	ampD	2465215..2465814 (+)	600	WP_054541864.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
LTQ54_RS11470 (LTQ54_11470)	pdhR	2466295..2467056 (+)	762	WP_004735446.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
LTQ54_RS11475 (LTQ54_11475)	aceE	2467119..2469782 (+)	2664	WP_004735447.1	pyruvate dehydrogenase (acetyl-transferring), homodimeric type	-
LTQ54_RS11480 (LTQ54_11480)	aceF	2469809..2471680 (+)	1872	WP_243578790.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-
LTQ54_RS11485 (LTQ54_11485)	lpdA	2471923..2473350 (+)	1428	WP_004735450.1	dihydrolipoyl dehydrogenase	-

Sequence

Protein

• Length: 561 a.a.
• Molecular weight: 62092.73 Da
• Isoelectric Point: 4.9258

>NTDB_id=635716 LTQ54_RS11450 WP_243578788.1 2461872..2463557(-) (pilB) [Vibrio splendidus strain 2_C04b]
MLTNLPTVLRQANLLSLTQEQAVAEHVQASGVSTPEALLVLDFFSGDSLANNIKTIFGLPLVRVANTDYEVLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEISDDEITSIEDLSQDDSPVSRFINQILVDAARKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCDHCKAADDSPDIFLRHSIPNSQI
IYKANSQGCNECNQGYSGRVGIYEVMPFSDQLKNSLIDKPNALAIENLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide

• Length: 1686 bp

>NTDB_id=635716 LTQ54_RS11450 WP_243578788.1 2461872..2463557(-) (pilB) [Vibrio splendidus strain 2_C04b]
ATGCTAACCAACCTCCCCACGGTTCTACGTCAGGCTAATCTACTTAGTCTGACTCAAGAACAAGCTGTTGCGGAACATGT
ACAAGCTTCAGGTGTTTCGACGCCTGAAGCTTTGCTCGTTCTTGATTTCTTCAGCGGTGATTCCTTAGCAAACAATATAA
AAACCATCTTCGGCTTACCGTTGGTACGAGTTGCTAATACAGATTATGAAGTATTATGTGAACAACTAGGGCTTCGCGAA
CTGATCACCAAGTATCGCGCTATTCCAATTTCAGTTTCTAGCTCAACTCTCACACTTGCCTCAGCCGACCCAACCGACTT
ACAAGCTGAAGACGACTTTCGATTCGCGACAGGATTACAGATTGAATTGGTTGTCGCTAATTACTCTGAATTAGAAGGCG
CAATACGAAAGCTGTATGGCCGTTCTATTTCCGGACAAGACTCCAAGCGTAAAGAGATCACTCAAGATGAGCTCGCTAAC
CTTGTTGAGATATCTGATGACGAGATAACTTCGATTGAAGACCTCAGCCAAGATGACTCCCCTGTCAGTCGCTTTATCAA
TCAGATTCTAGTCGATGCAGCACGTAAAGGCGCATCGGATATCCACTTCGAACCTTATGAAGAAAACTACCGCGTACGTC
TACGTTGCGATGGCATCCTTGTCGAAATTCAACAGCCCGCCTCTCATTTAAGTCGTCGCCTATCGGCTCGCTTGAAGATT
CTCGCCAAACTCGATATCGCCGAACGTCGCTTACCTCAAGATGGTCGTATCAAGTTACGTTTAAATGACGAACTCGCGAT
TGATATGCGTGTATCGACGCTGCCAACATTATGGGGAGAAAAGATCGTTCTTCGTCTTCTAGACAGCAGCGCTGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGATCAAAAAGCACTCTACCTCAATGCATTGAAACGCCCACAAGGGATGATC
TTAATGACCGGGCCAACGGGCAGCGGTAAAACGGTTTCTCTTTATACTGGCCTTCGAGTACTCAACACCACTGAACGCAA
TATATCAACAGCCGAAGACCCTGTTGAAATTAACCTATGTGGTATCAATCAGGTACAAGTGACGCCTAAGATCGGCTTTG
GTTTCGCTGAAGCGTTGCGATCATTTTTACGACAAGATCCCGATGTGGTAATGGTCGGTGAGATCCGCGATTTAGAAACC
GCAGAGATCGCGATCAAAGCATCGCAAACCGGACACCTCGTTCTTTCGACACTGCATACCAACTCCGCTGCAGAAACCGT
GACTCGACTCGCTCACATGGGAATTGAACCTTTTAATCTTGCTTCATCATTAAGCCTGATTATCGCCCAACGACTGGCAA
GACGATTGTGCGACCACTGTAAAGCCGCTGACGACTCACCTGATATATTTCTACGCCACTCTATTCCCAATAGCCAAATC
ATCTACAAAGCCAACTCACAAGGGTGTAATGAGTGTAATCAGGGATACTCGGGGCGAGTCGGTATCTATGAAGTCATGCC
GTTTAGCGACCAACTGAAAAATAGCCTGATAGACAAACCGAACGCGTTAGCCATTGAAAACTTGGCACGTCGAGAAGGCA
TGAGAACTTTACAAGAATCTGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (188-455)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Vibrio cholerae strain A1552	73.404	100	0.738
pilB	Vibrio campbellii strain DS40M4	70.285	100	0.704
pilB	Vibrio parahaemolyticus RIMD 2210633	69.751	100	0.699
pilB	Acinetobacter baumannii D1279779	50.482	92.513	0.467
pilB	Legionella pneumophila strain ERS1305867	50	90.909	0.455
pilB	Acinetobacter baylyi ADP1	48.94	92.513	0.453
pilF	Neisseria gonorrhoeae MS11	43.784	98.93	0.433