Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LTQ54_RS10050 Genome accession   NZ_CP089203
Coordinates   2206974..2207603 (+) Length   209 a.a.
NCBI ID   WP_004729667.1    Uniprot ID   -
Organism   Vibrio splendidus strain 2_C04b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2201974..2212603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTQ54_RS10035 (LTQ54_10035) - 2202660..2205221 (-) 2562 WP_181360097.1 PBP1A family penicillin-binding protein -
  LTQ54_RS10040 (LTQ54_10040) pilM 2205373..2206398 (+) 1026 WP_243578739.1 type IV pilus assembly protein PilM Machinery gene
  LTQ54_RS10045 (LTQ54_10045) pilN 2206382..2206981 (+) 600 WP_102488973.1 PilN domain-containing protein Machinery gene
  LTQ54_RS10050 (LTQ54_10050) pilO 2206974..2207603 (+) 630 WP_004729667.1 type 4a pilus biogenesis protein PilO Machinery gene
  LTQ54_RS10055 (LTQ54_10055) pilP 2207590..2208108 (+) 519 WP_004729666.1 pilus assembly protein PilP Machinery gene
  LTQ54_RS10060 (LTQ54_10060) pilQ 2208144..2209862 (+) 1719 WP_243578741.1 type IV pilus secretin PilQ family protein Machinery gene
  LTQ54_RS10065 (LTQ54_10065) aroK 2210039..2210557 (+) 519 WP_004729664.1 shikimate kinase AroK -
  LTQ54_RS10070 (LTQ54_10070) aroB 2210583..2211671 (+) 1089 WP_004729663.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 24294.86 Da        Isoelectric Point: 4.6751

>NTDB_id=635703 LTQ54_RS10050 WP_004729667.1 2206974..2207603(+) (pilO) [Vibrio splendidus strain 2_C04b]
MANLQNKISWQNRMSLQDLDVDEITEWPLLPQLLVILVLIVLIQGVGTWLYVLPLDDELQQMKQQEQTLKATLRIKANKV
AALPKLQSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNKLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGD
FSAAIAKLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDETQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=635703 LTQ54_RS10050 WP_004729667.1 2206974..2207603(+) (pilO) [Vibrio splendidus strain 2_C04b]
ATGGCTAACCTTCAAAATAAGATTAGTTGGCAAAACAGGATGAGTTTGCAAGATCTCGATGTTGATGAGATCACCGAATG
GCCATTGTTGCCTCAGCTTCTCGTGATTCTGGTGTTGATAGTGTTAATCCAAGGTGTTGGCACTTGGCTTTATGTGCTGC
CGCTCGACGATGAACTGCAACAGATGAAGCAACAGGAGCAGACCTTAAAAGCGACCTTGAGAATAAAGGCCAATAAGGTT
GCGGCCTTACCTAAGCTTCAGAGCCAGTTGGATGAGCTAACTAGCCGTTACGACTATCTATTGGAGCAATTGCCCGTTCA
AAAAGAGCTCGCCAGTATGTTGGCCTCTGTGAACGAGCTTGGATTGGATAATAAGCTGACATTTACGCGAATTGACTGGG
GGCAGAAGCAGAACAAAGAGTTTCTCTATCGTTTACCGCTCAACATCGAGCTGACGGGTGACTACCACGAGATTGGTGAT
TTCTCTGCGGCCATTGCTAAGCTGCCACGCATCATCAGCTTTGATGACGTGAATTGGCAGCGAGTCAGTCAAGAAAGTAG
CACGCTGCACTTCAGGGTTCGAGCTTATACCTACCAATTTAAATCGGAGGTCAATGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(59-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

76.166

92.344

0.703
  pilO Vibrio campbellii strain DS40M4

73.514

88.517

0.651