Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   LTQ54_RS10045 Genome accession   NZ_CP089203
Coordinates   2206382..2206981 (+) Length   199 a.a.
NCBI ID   WP_102488973.1    Uniprot ID   -
Organism   Vibrio splendidus strain 2_C04b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2201382..2211981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTQ54_RS10025 (LTQ54_10025) - 2201476..2201655 (-) 180 WP_017077611.1 hypothetical protein -
  LTQ54_RS10030 (LTQ54_10030) oxyR 2201654..2202547 (+) 894 WP_017080198.1 DNA-binding transcriptional regulator OxyR -
  LTQ54_RS10035 (LTQ54_10035) - 2202660..2205221 (-) 2562 WP_181360097.1 PBP1A family penicillin-binding protein -
  LTQ54_RS10040 (LTQ54_10040) pilM 2205373..2206398 (+) 1026 WP_243578739.1 type IV pilus assembly protein PilM Machinery gene
  LTQ54_RS10045 (LTQ54_10045) pilN 2206382..2206981 (+) 600 WP_102488973.1 PilN domain-containing protein Machinery gene
  LTQ54_RS10050 (LTQ54_10050) pilO 2206974..2207603 (+) 630 WP_004729667.1 type 4a pilus biogenesis protein PilO Machinery gene
  LTQ54_RS10055 (LTQ54_10055) pilP 2207590..2208108 (+) 519 WP_004729666.1 pilus assembly protein PilP Machinery gene
  LTQ54_RS10060 (LTQ54_10060) pilQ 2208144..2209862 (+) 1719 WP_243578741.1 type IV pilus secretin PilQ family protein Machinery gene
  LTQ54_RS10065 (LTQ54_10065) aroK 2210039..2210557 (+) 519 WP_004729664.1 shikimate kinase AroK -
  LTQ54_RS10070 (LTQ54_10070) aroB 2210583..2211671 (+) 1089 WP_004729663.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22984.47 Da        Isoelectric Point: 9.8078

>NTDB_id=635702 LTQ54_RS10045 WP_102488973.1 2206382..2206981(+) (pilN) [Vibrio splendidus strain 2_C04b]
MLHQINLLPWRDEIRAQHKKRFIHLIILGVIIALAGQWAVGHYFYDQQAKQQARLSYLNRYIAELDRQIQSLKVAEQEHK
AILTRLDVVESLQLGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKGVEMHSIV
HNRKRFNKEFQTFKVSFVFSPVSLENTTASRKEKGAHHG

Nucleotide


Download         Length: 600 bp        

>NTDB_id=635702 LTQ54_RS10045 WP_102488973.1 2206382..2206981(+) (pilN) [Vibrio splendidus strain 2_C04b]
ATGTTGCATCAAATTAACCTGCTGCCGTGGCGTGATGAGATTCGCGCTCAGCACAAGAAGCGCTTTATCCATCTGATCAT
TCTTGGCGTCATCATCGCGCTTGCAGGGCAATGGGCGGTTGGTCACTATTTTTACGACCAACAAGCCAAGCAGCAAGCCC
GTCTTAGTTACCTCAATCGATATATTGCCGAGCTCGACCGACAAATTCAGTCATTAAAGGTCGCAGAGCAGGAGCATAAA
GCGATTTTGACTAGGCTTGATGTAGTCGAATCGTTGCAGCTTGGTCGCAACAAGACCACCGACTTTATGAACCTGATGCC
GGAGCTGATTCCTGAGGGTGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATTAGCGACA
GTACTGCTCGTCTCGCTACGATGTTGGACAACCTCGAGCGTTCAGATTCTCTGAAAGGGGTCGAGATGCACTCTATCGTT
CATAACCGCAAGCGCTTCAATAAAGAGTTTCAGACCTTCAAGGTCTCTTTTGTGTTTAGCCCTGTGTCTTTAGAAAACAC
AACCGCGAGCAGAAAAGAGAAGGGGGCGCATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

62.5

100

0.628
  pilN Vibrio campbellii strain DS40M4

63.978

93.467

0.598