Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LTK17_RS12735 Genome accession   NZ_CP089157
Coordinates   2633845..2634342 (-) Length   165 a.a.
NCBI ID   WP_001794055.1    Uniprot ID   A0A7U7EV69
Organism   Staphylococcus aureus strain UNC_SaCF30     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2628845..2639342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTK17_RS12710 (LTK17_12710) gcvPB 2629007..2630479 (-) 1473 WP_209055752.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  LTK17_RS12715 (LTK17_12715) gcvPA 2630472..2631818 (-) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  LTK17_RS12720 (LTK17_12720) gcvT 2631838..2632929 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  LTK17_RS12725 (LTK17_12725) - 2633088..2633612 (-) 525 WP_001015125.1 shikimate kinase -
  LTK17_RS12730 (LTK17_12730) - 2633602..2633748 (-) 147 WP_001789879.1 hypothetical protein -
  LTK17_RS12735 (LTK17_12735) comGF 2633845..2634342 (-) 498 WP_001794055.1 competence type IV pilus minor pilin ComGF Machinery gene
  LTK17_RS12740 (LTK17_12740) comGE 2634260..2634559 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  LTK17_RS12745 (LTK17_12745) comGD 2634546..2634992 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  LTK17_RS12750 (LTK17_12750) comGC 2634970..2635281 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  LTK17_RS12755 (LTK17_12755) comGB 2635295..2636365 (-) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  LTK17_RS12760 (LTK17_12760) comGA 2636337..2637311 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  LTK17_RS12765 (LTK17_12765) - 2637363..2637986 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  LTK17_RS12770 (LTK17_12770) - 2637983..2638312 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  LTK17_RS12775 (LTK17_12775) - 2638312..2639298 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19224.98 Da        Isoelectric Point: 10.2192

>NTDB_id=635455 LTK17_RS12735 WP_001794055.1 2633845..2634342(-) (comGF) [Staphylococcus aureus strain UNC_SaCF30]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLNKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=635455 LTK17_RS12735 WP_001794055.1 2633845..2634342(-) (comGF) [Staphylococcus aureus strain UNC_SaCF30]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAACAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EV69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97