NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	LRO88_RS14235	Genome accession	NZ_CP089148
Coordinates	2603665..2604282 (-)	Length	205 a.a.
NCBI ID	WP_032726193.1	Uniprot ID	-
Organism	Bacillus subtilis strain NIB353
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2598665..2609282

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRO88_RS14205	rpsT	2598965..2599231 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
LRO88_RS14210	holA	2599246..2600289 (-)	1044	WP_159376962.1	DNA polymerase III subunit delta	-
LRO88_RS14215	-	2600329..2600442 (-)	114	WP_134975484.1	hypothetical protein	-
LRO88_RS14220	yqzM	2600519..2600653 (+)	135	WP_003229983.1	YqzM family protein	-
LRO88_RS14225	comEC	2600695..2603025 (-)	2331	WP_159376963.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LRO88_RS14230	comEB	2603029..2603598 (-)	570	WP_003229978.1	ComE operon protein 2	-
LRO88_RS14235	comEA	2603665..2604282 (-)	618	WP_032726193.1	competence protein ComEA	Machinery gene
LRO88_RS14240	comER	2604366..2605187 (+)	822	WP_159376964.1	late competence protein ComER	-
LRO88_RS14245	yqeM	2605253..2605996 (-)	744	WP_032726197.1	class I SAM-dependent DNA methyltransferase	-
LRO88_RS14250	rsfS	2605993..2606349 (-)	357	WP_014480315.1	ribosome silencing factor	-
LRO88_RS14255	yqeK	2606367..2606927 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LRO88_RS14260	nadD	2606917..2607486 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
LRO88_RS14265	yhbY	2607498..2607788 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
LRO88_RS14270	aroE	2607782..2608624 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 205 a.a. Molecular weight: 21753.50 Da Isoelectric Point: 4.8417

                                >NTDB_id=635316 LRO88_RS14235 WP_032726193.1 2603665..2604282(-) (comEA) [Bacillus subtilis strain NIB353]

MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT

GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV

GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide

Download Length: 618 bp

                                >NTDB_id=635316 LRO88_RS14235 WP_032726193.1 2603665..2604282(-) (comEA) [Bacillus subtilis strain NIB353]

ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT

GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA

ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA

GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA

GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG

TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG

GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT

TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376

Detailed information NTG0635316