Detailed information NTG0635125

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LSP21_RS08975
• Genome accession: NZ_CP089068
• Coordinates: 1954322..1955470 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1949322..1960470

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSP21_RS08950 (LSP21_08960)	-	1950473..1950769 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
LSP21_RS08955 (LSP21_08965)	-	1950766..1951359 (-)	594	WP_003084544.1	YggT family protein	-
LSP21_RS08960 (LSP21_08970)	proC	1951370..1952191 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LSP21_RS08965 (LSP21_08975)	-	1952203..1952895 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
LSP21_RS08970 (LSP21_08980)	pilT	1953110..1954144 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LSP21_RS08975 (LSP21_08985)	pilU	1954322..1955470 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
LSP21_RS08980 (LSP21_08990)	czcD	1955478..1956377 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LSP21_RS08985 (LSP21_08995)	-	1956531..1956935 (+)	405	WP_003084560.1	NINE protein	-
LSP21_RS08990 (LSP21_09000)	-	1957178..1958551 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
LSP21_RS08995 (LSP21_09005)	-	1958548..1959732 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=635125 LSP21_RS08975 WP_003100959.1 1954322..1955470(+) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF13]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=635125 LSP21_RS08975 WP_003100959.1 1954322..1955470(+) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF13]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382