Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LSP16_RS07720 Genome accession   NZ_CP089067
Coordinates   1607042..1608106 (+) Length   354 a.a.
NCBI ID   WP_219832833.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain UNC_PaerCF19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1602042..1613106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSP16_RS07710 (LSP16_07700) - 1603025..1604293 (+) 1269 WP_003095842.1 malic enzyme -
  LSP16_RS07715 (LSP16_07705) - 1604389..1606857 (-) 2469 WP_003114576.1 penicillin-binding protein 1A -
  LSP16_RS07720 (LSP16_07710) pilM 1607042..1608106 (+) 1065 WP_219832833.1 type IV pilus biogenesis protein PilM Machinery gene
  LSP16_RS07725 (LSP16_07715) pilN 1608106..1608702 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  LSP16_RS07730 (LSP16_07720) pilO 1608699..1609322 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  LSP16_RS07735 (LSP16_07725) pilP 1609319..1609843 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  LSP16_RS07740 (LSP16_07730) pilQ 1609897..1612041 (+) 2145 WP_219832834.1 type IV pilus secretin PilQ Machinery gene
  LSP16_RS07745 (LSP16_07735) aroK 1612053..1612571 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37967.44 Da        Isoelectric Point: 4.5405

>NTDB_id=635085 LSP16_RS07720 WP_219832833.1 1607042..1608106(+) (pilM) [Pseudomonas aeruginosa strain UNC_PaerCF19]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSPQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=635085 LSP16_RS07720 WP_219832833.1 1607042..1608106(+) (pilM) [Pseudomonas aeruginosa strain UNC_PaerCF19]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCCGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.618

100

0.559
  comM Acinetobacter nosocomialis M2

55.618

100

0.559
  comM Acinetobacter baylyi ADP1

51.695

100

0.517
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463