Detailed information NTG0635042

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LSP20_RS01395
• Genome accession: NZ_CP089065
• Coordinates: 287716..288864 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF34
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 282716..293864

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSP20_RS01375 (LSP20_01375)	-	283454..284638 (-)	1185	WP_053870615.1	cystathionine gamma-synthase	-
LSP20_RS01380 (LSP20_01380)	-	284635..286008 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
LSP20_RS01385 (LSP20_01385)	-	286251..286655 (-)	405	WP_003084560.1	NINE protein	-
LSP20_RS01390 (LSP20_01390)	czcD	286809..287708 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LSP20_RS01395 (LSP20_01395)	pilU	287716..288864 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
LSP20_RS01400 (LSP20_01400)	pilT	289042..290076 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LSP20_RS01405 (LSP20_01405)	-	290291..290983 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
LSP20_RS01410 (LSP20_01410)	proC	290995..291816 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LSP20_RS01415 (LSP20_01415)	-	291827..292420 (+)	594	WP_003084544.1	YggT family protein	-
LSP20_RS01420 (LSP20_01420)	-	292417..292713 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=635042 LSP20_RS01395 WP_003100959.1 287716..288864(-) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF34]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=635042 LSP20_RS01395 WP_003100959.1 287716..288864(-) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF34]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382