Detailed information NTG0635003

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LSP18_RS29390
• Genome accession: NZ_CP089063
• Coordinates: 6294558..6295706 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF37
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6289558..6300706

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSP18_RS29370 (LSP18_29625)	-	6290295..6291479 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
LSP18_RS29375 (LSP18_29630)	-	6291476..6292849 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
LSP18_RS29380 (LSP18_29635)	-	6293092..6293496 (-)	405	WP_003084560.1	NINE protein	-
LSP18_RS29385 (LSP18_29640)	czcD	6293651..6294550 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LSP18_RS29390 (LSP18_29645)	pilU	6294558..6295706 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
LSP18_RS29395 (LSP18_29650)	pilT	6295884..6296918 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LSP18_RS29400 (LSP18_29655)	-	6297133..6297825 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
LSP18_RS29405 (LSP18_29660)	proC	6297837..6298658 (+)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
LSP18_RS29410 (LSP18_29665)	-	6298669..6299262 (+)	594	WP_003084544.1	YggT family protein	-
LSP18_RS29415 (LSP18_29670)	-	6299259..6299555 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=635003 LSP18_RS29390 WP_003100959.1 6294558..6295706(-) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF37]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=635003 LSP18_RS29390 WP_003100959.1 6294558..6295706(-) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF37]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382