Detailed information NTG0634946

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LSP17_RS07680
• Genome accession: NZ_CP089062
• Coordinates: 1662046..1663194 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF38
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1657046..1668194

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSP17_RS07655 (LSP17_07655)	-	1658197..1658493 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
LSP17_RS07660 (LSP17_07660)	-	1658490..1659083 (-)	594	WP_003084544.1	YggT family protein	-
LSP17_RS07665 (LSP17_07665)	proC	1659094..1659915 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LSP17_RS07670 (LSP17_07670)	-	1659927..1660619 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
LSP17_RS07675 (LSP17_07675)	pilT	1660834..1661868 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LSP17_RS07680 (LSP17_07680)	pilU	1662046..1663194 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
LSP17_RS07685 (LSP17_07685)	czcD	1663202..1664101 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LSP17_RS07690 (LSP17_07690)	-	1664255..1664659 (+)	405	WP_003084560.1	NINE protein	-
LSP17_RS07695 (LSP17_07695)	-	1664902..1666275 (+)	1374	WP_073669068.1	pyridoxal-phosphate dependent enzyme	-
LSP17_RS07700 (LSP17_07700)	-	1666272..1667456 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=634946 LSP17_RS07680 WP_003100959.1 1662046..1663194(+) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF38]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=634946 LSP17_RS07680 WP_003100959.1 1662046..1663194(+) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF38]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382