Detailed information NTG0634377

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LRK52_RS04245
• Genome accession: NZ_CP088980
• Coordinates: 950894..952060 (+)
• Length: 388 a.a.
• NCBI ID: WP_015446994.1
• Uniprot ID: -
• Organism: Rhodanobacter denitrificans strain FW104-R5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 945894..957060

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRK52_RS04220 (LRK52_04220)	-	946014..946454 (+)	441	WP_015446991.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
LRK52_RS04225 (LRK52_04225)	-	946459..947283 (+)	825	WP_037115662.1	cobalamin-binding protein	-
LRK52_RS04230 (LRK52_04230)	-	947938..948513 (-)	576	WP_007507063.1	YggT family protein	-
LRK52_RS04235 (LRK52_04235)	proC	948510..949334 (-)	825	WP_015446993.1	pyrroline-5-carboxylate reductase	-
LRK52_RS04240 (LRK52_04240)	pilT	949703..950740 (+)	1038	WP_007507061.1	type IV pilus twitching motility protein PilT	Machinery gene
LRK52_RS04245 (LRK52_04245)	pilU	950894..952060 (+)	1167	WP_015446994.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LRK52_RS04250 (LRK52_04250)	-	952127..952504 (-)	378	WP_015446995.1	BON domain-containing protein	-
LRK52_RS04255 (LRK52_04255)	-	952857..955067 (-)	2211	WP_231378422.1	TonB-dependent siderophore receptor	-
LRK52_RS04260 (LRK52_04260)	-	955213..955905 (+)	693	WP_015446997.1	response regulator transcription factor	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 43408.83 Da
• Isoelectric Point: 6.7735

>NTDB_id=634377 LRK52_RS04245 WP_015446994.1 950894..952060(+) (pilU) [Rhodanobacter denitrificans strain FW104-R5]
MSDFDFTSFLKLMVHKKASDLFITAGVAPSMKVQGRIVPITQSPLSVQQARDMVLNVMTPGQREEFEKTHECQFAISAQG
VGRFRVSCFYQRNCVGMVLRRIESKIPTIEELTLPPVIKQLAMTKRGIIVFVGATGSGKSTSLASMIGYRNQNSTGHIIT
IEDPIEYVHKHEGCIITQREVGIDTDSWDNALKNTLRQAPDVIMIGEIRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRILHFFPEDRRQQLLMDLSLNLKGIVAQQLIPTPDGKARRVAVEILLGTPLVQDYIRQGEIHKLKEVMKESTLLGMKTF
DQSLVELYHAGEISYEDALRYADSSNEVRLRIKLAQGGDAHTLSQGLEGVELEESRDAQNFSSNFLNR

Nucleotide

• Length: 1167 bp

>NTDB_id=634377 LRK52_RS04245 WP_015446994.1 950894..952060(+) (pilU) [Rhodanobacter denitrificans strain FW104-R5]
ATGAGCGACTTCGATTTCACCTCATTCCTGAAGCTGATGGTGCACAAGAAGGCCTCCGACTTGTTCATCACGGCGGGCGT
CGCCCCCTCGATGAAAGTGCAGGGCCGGATCGTGCCGATCACGCAGAGCCCGCTCAGCGTGCAGCAGGCGCGCGACATGG
TGCTCAACGTGATGACGCCGGGCCAGCGCGAGGAGTTCGAGAAGACCCATGAGTGCCAGTTCGCGATCTCCGCGCAAGGC
GTGGGCCGTTTCCGCGTGTCGTGCTTCTACCAGCGCAACTGCGTGGGTATGGTGCTGCGCCGGATCGAGTCGAAGATCCC
CACCATCGAGGAACTGACGCTGCCGCCGGTGATCAAGCAACTGGCGATGACCAAGCGCGGCATCATCGTGTTCGTCGGCG
CCACCGGCTCGGGCAAATCGACCTCGCTGGCGTCGATGATCGGCTACCGCAACCAGAACTCGACCGGTCATATCATCACG
ATCGAGGACCCGATCGAGTACGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGACAACGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCATGATCGGCGAAATCCGCACCCGCGAGGGCA
TGGACCACGCGATCGCCTTTGCCGAAACCGGTCACCTGGTGCTGTGCACGCTGCACGCGAACAACGCCAACCAGGCGATG
GACCGCATCCTGCACTTCTTCCCCGAGGACCGCCGCCAGCAACTGCTGATGGACCTGTCGCTCAACCTGAAGGGCATCGT
GGCGCAGCAGCTGATCCCCACGCCCGACGGCAAGGCGCGCCGGGTCGCGGTGGAGATTCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCCAGGGCGAGATCCACAAGCTGAAGGAGGTGATGAAGGAGTCCACTCTGCTCGGCATGAAGACCTTC
GACCAGAGCCTGGTCGAGCTGTACCACGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCTACGCCGACAGCTCCAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCGCATACCCTGTCGCAAGGCCTGGAAGGCGTCGAGCTGGAGG
AAAGCCGCGACGCGCAGAACTTCAGCAGCAATTTCCTCAACCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	66.578	96.392	0.642
pilU	Acinetobacter baylyi ADP1	63.483	91.753	0.582
pilU	Vibrio cholerae strain A1552	52.809	91.753	0.485
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.129	95.876	0.394