NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	LSG23_RS12560	Genome accession	NZ_CP088973
Coordinates	2583952..2585022 (-)	Length	356 a.a.
NCBI ID	WP_070082113.1	Uniprot ID	-
Organism	Bacillus velezensis strain CMML20-16
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2578952..2590022

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSG23_RS12510 (LSG23_12490)	sipW	2579012..2579596 (-)	585	WP_012117977.1	signal peptidase I SipW	-
LSG23_RS12515 (LSG23_12495)	tapA	2579568..2580239 (-)	672	WP_070082109.1	amyloid fiber anchoring/assembly protein TapA	-
LSG23_RS12520 (LSG23_12500)	-	2580498..2580827 (+)	330	WP_003153097.1	DUF3889 domain-containing protein	-
LSG23_RS12525 (LSG23_12505)	-	2580867..2581046 (-)	180	WP_003153093.1	YqzE family protein	-
LSG23_RS12530 (LSG23_12510)	comGG	2581103..2581480 (-)	378	WP_077722592.1	competence type IV pilus minor pilin ComGG	Machinery gene
LSG23_RS12535 (LSG23_12515)	comGF	2581481..2581981 (-)	501	WP_232213409.1	competence type IV pilus minor pilin ComGF	-
LSG23_RS12540 (LSG23_12520)	comGE	2581890..2582204 (-)	315	WP_077722594.1	competence type IV pilus minor pilin ComGE	Machinery gene
LSG23_RS12545 (LSG23_12525)	comGD	2582188..2582625 (-)	438	WP_077722595.1	competence type IV pilus minor pilin ComGD	Machinery gene
LSG23_RS12550 (LSG23_12530)	comGC	2582615..2582923 (-)	309	WP_003153087.1	competence type IV pilus major pilin ComGC	Machinery gene
LSG23_RS12555 (LSG23_12535)	comGB	2582928..2583965 (-)	1038	WP_063174751.1	competence type IV pilus assembly protein ComGB	Machinery gene
LSG23_RS12560 (LSG23_12540)	comGA	2583952..2585022 (-)	1071	WP_070082113.1	competence type IV pilus ATPase ComGA	Machinery gene
LSG23_RS12565 (LSG23_12545)	-	2585214..2586164 (-)	951	WP_003153082.1	magnesium transporter CorA family protein	-
LSG23_RS12570 (LSG23_12550)	-	2586310..2587611 (+)	1302	WP_077722596.1	hemolysin family protein	-
LSG23_RS12575 (LSG23_12555)	-	2587649..2588479 (-)	831	WP_070082115.1	STAS domain-containing protein	-
LSG23_RS12585 (LSG23_12565)	-	2588685..2589065 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
LSG23_RS12590 (LSG23_12570)	-	2589302..2589547 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40557.91 Da Isoelectric Point: 9.5862

                                >NTDB_id=634311 LSG23_RS12560 WP_070082113.1 2583952..2585022(-) (comGA) [Bacillus velezensis strain CMML20-16]

MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFREAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=634311 LSG23_RS12560 WP_070082113.1 2583952..2585022(-) (comGA) [Bacillus velezensis strain CMML20-16]

GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT

GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC

ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC

CATCGCTGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCGGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	81.742	100	0.817
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0634311