Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LSG23_RS12555 Genome accession   NZ_CP088973
Coordinates   2582928..2583965 (-) Length   345 a.a.
NCBI ID   WP_063174751.1    Uniprot ID   -
Organism   Bacillus velezensis strain CMML20-16     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2577928..2588965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSG23_RS12505 (LSG23_12485) tasA 2578163..2578948 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  LSG23_RS12510 (LSG23_12490) sipW 2579012..2579596 (-) 585 WP_012117977.1 signal peptidase I SipW -
  LSG23_RS12515 (LSG23_12495) tapA 2579568..2580239 (-) 672 WP_070082109.1 amyloid fiber anchoring/assembly protein TapA -
  LSG23_RS12520 (LSG23_12500) - 2580498..2580827 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  LSG23_RS12525 (LSG23_12505) - 2580867..2581046 (-) 180 WP_003153093.1 YqzE family protein -
  LSG23_RS12530 (LSG23_12510) comGG 2581103..2581480 (-) 378 WP_077722592.1 competence type IV pilus minor pilin ComGG Machinery gene
  LSG23_RS12535 (LSG23_12515) comGF 2581481..2581981 (-) 501 WP_232213409.1 competence type IV pilus minor pilin ComGF -
  LSG23_RS12540 (LSG23_12520) comGE 2581890..2582204 (-) 315 WP_077722594.1 competence type IV pilus minor pilin ComGE Machinery gene
  LSG23_RS12545 (LSG23_12525) comGD 2582188..2582625 (-) 438 WP_077722595.1 competence type IV pilus minor pilin ComGD Machinery gene
  LSG23_RS12550 (LSG23_12530) comGC 2582615..2582923 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  LSG23_RS12555 (LSG23_12535) comGB 2582928..2583965 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  LSG23_RS12560 (LSG23_12540) comGA 2583952..2585022 (-) 1071 WP_070082113.1 competence type IV pilus ATPase ComGA Machinery gene
  LSG23_RS12565 (LSG23_12545) - 2585214..2586164 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  LSG23_RS12570 (LSG23_12550) - 2586310..2587611 (+) 1302 WP_077722596.1 hemolysin family protein -
  LSG23_RS12575 (LSG23_12555) - 2587649..2588479 (-) 831 WP_070082115.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39558.87 Da        Isoelectric Point: 9.4989

>NTDB_id=634310 LSG23_RS12555 WP_063174751.1 2582928..2583965(-) (comGB) [Bacillus velezensis strain CMML20-16]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYEVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=634310 LSG23_RS12555 WP_063174751.1 2582928..2583965(-) (comGB) [Bacillus velezensis strain CMML20-16]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGTGATGCGTTTTACGAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCAGAGAAACACGGAGAATTGCCAGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCGTTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTCATTGTTGTGAAAATACCCCTCTTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGTTATGAAAAAGATCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614