Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SMAL454_RS05610 Genome accession   NZ_CP088957
Coordinates   1180582..1181724 (+) Length   380 a.a.
NCBI ID   WP_005408369.1    Uniprot ID   A0A854DW57
Organism   Stenotrophomonas maltophilia strain 454     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1175582..1186724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMAL454_RS05580 (SMAL454_11110) soxR 1175696..1176130 (+) 435 WP_005412484.1 redox-sensitive transcriptional activator SoxR -
  SMAL454_RS05585 (SMAL454_11120) - 1176203..1176805 (-) 603 WP_062606615.1 short chain dehydrogenase -
  SMAL454_RS05590 (SMAL454_11130) - 1176904..1177779 (+) 876 WP_060381038.1 LysR family transcriptional regulator -
  SMAL454_RS05595 (SMAL454_11140) proC 1177839..1178660 (-) 822 WP_154329277.1 pyrroline-5-carboxylate reductase -
  SMAL454_RS05600 (SMAL454_11150) - 1178679..1179356 (-) 678 WP_005412486.1 YggS family pyridoxal phosphate-dependent enzyme -
  SMAL454_RS05605 (SMAL454_11160) pilT 1179444..1180481 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  SMAL454_RS05610 (SMAL454_11170) pilU 1180582..1181724 (+) 1143 WP_005408369.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SMAL454_RS05615 (SMAL454_11180) - 1181932..1182915 (+) 984 WP_154329278.1 aldo/keto reductase -
  SMAL454_RS05620 (SMAL454_11190) - 1182963..1183862 (-) 900 WP_099484780.1 LysR family transcriptional regulator -
  SMAL454_RS05625 (SMAL454_11200) - 1184191..1184403 (+) 213 WP_043034209.1 DUF4287 domain-containing protein -
  SMAL454_RS05630 (SMAL454_11210) - 1184514..1185221 (+) 708 WP_014036228.1 YitT family protein -
  SMAL454_RS05635 (SMAL454_11220) - 1185305..1185850 (-) 546 WP_049451061.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42355.69 Da        Isoelectric Point: 6.7683

>NTDB_id=634235 SMAL454_RS05610 WP_005408369.1 1180582..1181724(+) (pilU) [Stenotrophomonas maltophilia strain 454]
MNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLG
MKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=634235 SMAL454_RS05610 WP_005408369.1 1180582..1181724(+) (pilU) [Stenotrophomonas maltophilia strain 454]
GTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGACCTGTTCAT
CACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGACCCCGCTCACGCCGCAGCAGA
GCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAATGCAACTTCGCCATC
GGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGCATCGA
GACGCGCATTCCGACGGTGGAAGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTCGGTGCCACCGGTACCGGTAAATCGACGTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAACTCGACCGGC
CACATCATCACCATCGAAGATCCGATTGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCAT
CGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTGCGTA
CCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCATGCCAACAACGCC
AACCAGGCGATGGACCGCATCGTCAATTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGACCTGTCGCTGAATCT
CAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTCGCGCAAGGTGGCGATGGAGATCCTGCTGGGCA
CGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGACTCGGTCCAGCTGGGC
ATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGTTACGCCGA
TTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCACGCACGCTGTCACAGGGCCTGGATGGCG
TGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A854DW57

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

96.579

0.655
  pilU Acinetobacter baylyi ADP1

63.585

93.947

0.597
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

93.421

0.403