Detailed information NTG0634058

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA6_RS02030
• Genome accession: NZ_CP104867
• Coordinates: 436629..437777 (+)
• Length: 382 a.a.
• NCBI ID: WP_269975408.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain PALA6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 431629..442777

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA6_RS02005	-	432780..433076 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PALA6_RS02010 (PALA6_00406)	-	433073..433666 (-)	594	WP_003084544.1	YggT family protein	-
PALA6_RS02015 (PALA6_00407)	proC	433677..434498 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
PALA6_RS02020 (PALA6_00408)	-	434510..435202 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA6_RS02025 (PALA6_00409)	pilT	435417..436451 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA6_RS02030 (PALA6_00410)	pilU	436629..437777 (+)	1149	WP_269975408.1	type IV pilus ATPase PilU	Machinery gene
PALA6_RS02035 (PALA6_00411)	czcD	437785..438684 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PALA6_RS02040 (PALA6_00412)	-	438839..439243 (+)	405	WP_003084560.1	NINE protein	-
PALA6_RS02045 (PALA6_00413)	-	439486..440859 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
PALA6_RS02050 (PALA6_00414)	-	440856..442040 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42544.86 Da
• Isoelectric Point: 6.7792

>NTDB_id=634058 PALA6_RS02030 WP_269975408.1 436629..437777(+) (pilU) [Pseudomonas aeruginosa strain PALA6]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLIQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=634058 PALA6_RS02030 WP_269975408.1 436629..437777(+) (pilU) [Pseudomonas aeruginosa strain PALA6]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGATCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	57.682	97.12	0.56
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382