Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PALA4_RS28980 Genome accession   NZ_CP104866
Coordinates   6242356..6242979 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PALA4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6237356..6247979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA4_RS28960 (PALA4_05721) aroB 6237951..6239057 (-) 1107 WP_043091242.1 3-dehydroquinate synthase -
  PALA4_RS28965 (PALA4_05722) aroK 6239107..6239625 (-) 519 WP_003095833.1 shikimate kinase AroK -
  PALA4_RS28970 (PALA4_05723) pilQ 6239637..6241781 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  PALA4_RS28975 (PALA4_05724) comL/pilP 6241835..6242359 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PALA4_RS28980 (PALA4_05725) pilO 6242356..6242979 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PALA4_RS28985 (PALA4_05726) pilN 6242976..6243572 (-) 597 WP_043091243.1 type 4a pilus biogenesis protein PilN Machinery gene
  PALA4_RS28990 (PALA4_05727) pilM 6243572..6244636 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PALA4_RS28995 (PALA4_05728) - 6244821..6247292 (+) 2472 WP_003110889.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=634032 PALA4_RS28980 WP_003103268.1 6242356..6242979(-) (pilO) [Pseudomonas aeruginosa strain PALA4]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=634032 PALA4_RS28980 WP_003103268.1 6242356..6242979(-) (pilO) [Pseudomonas aeruginosa strain PALA4]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTTGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAACTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401