Detailed information NTG0633812

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LRX86_RS14015
• Genome accession: NZ_CP088895
• Coordinates: 2949812..2950930 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain DETAB-R21
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2944812..2955930

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRX86_RS14005 (LRX86_14005)	-	2947928..2948620 (-)	693	WP_002124845.1	YggS family pyridoxal phosphate-dependent enzyme	-
LRX86_RS14010 (LRX86_14010)	pilT	2948747..2949784 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
LRX86_RS14015 (LRX86_14015)	pilU	2949812..2950930 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LRX86_RS14020 (LRX86_14020)	fur	2951000..2951437 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
LRX86_RS14025 (LRX86_14025)	-	2951549..2951947 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
LRX86_RS14030 (LRX86_14030)	-	2952002..2952325 (-)	324	WP_000442589.1	RnfH family protein	-
LRX86_RS14035 (LRX86_14035)	-	2952325..2953392 (-)	1068	WP_004835750.1	hypothetical protein	-
LRX86_RS14040 (LRX86_14040)	-	2953545..2954000 (-)	456	WP_029574892.1	bacteriohemerythrin	-
LRX86_RS14045 (LRX86_14045)	-	2954253..2955101 (-)	849	WP_029574891.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=633812 LRX86_RS14015 WP_000347036.1 2949812..2950930(+) (pilU) [Acinetobacter baumannii strain DETAB-R21]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=633812 LRX86_RS14015 WP_000347036.1 2949812..2950930(+) (pilU) [Acinetobacter baumannii strain DETAB-R21]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392