Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LPC09_RS17835 Genome accession   NZ_CP088245
Coordinates   3500545..3501177 (-) Length   210 a.a.
NCBI ID   WP_231307912.1    Uniprot ID   -
Organism   Metabacillus sp. B2-18     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3495545..3506177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPC09_RS17815 (LPC09_17815) holA 3495894..3496916 (-) 1023 WP_231307909.1 DNA polymerase III subunit delta -
  LPC09_RS17820 (LPC09_17820) - 3497301..3497435 (+) 135 WP_121662901.1 YqzM family protein -
  LPC09_RS17825 (LPC09_17825) - 3497488..3499752 (-) 2265 WP_231307910.1 DNA internalization-related competence protein ComEC/Rec2 -
  LPC09_RS17830 (LPC09_17830) - 3499903..3500460 (-) 558 WP_231307911.1 ComE operon protein 2 -
  LPC09_RS17835 (LPC09_17835) comEA 3500545..3501177 (-) 633 WP_231307912.1 helix-hairpin-helix domain-containing protein Machinery gene
  LPC09_RS17840 (LPC09_17840) comER 3501268..3502101 (+) 834 WP_231307913.1 late competence protein ComER -
  LPC09_RS17845 (LPC09_17845) - 3502163..3502921 (-) 759 WP_231307914.1 class I SAM-dependent methyltransferase -
  LPC09_RS17850 (LPC09_17850) rsfS 3502918..3503274 (-) 357 WP_231307915.1 ribosome silencing factor -
  LPC09_RS17855 (LPC09_17855) yqeK 3503274..3503846 (-) 573 WP_231307916.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  LPC09_RS17860 (LPC09_17860) - 3503836..3504408 (-) 573 WP_231307917.1 nicotinate-nucleotide adenylyltransferase -
  LPC09_RS17865 (LPC09_17865) yhbY 3504427..3504720 (-) 294 WP_231307918.1 ribosome assembly RNA-binding protein YhbY -
  LPC09_RS17870 (LPC09_17870) aroE 3504714..3505556 (-) 843 WP_231307919.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 23031.07 Da        Isoelectric Point: 4.3860

>NTDB_id=633479 LPC09_RS17835 WP_231307912.1 3500545..3501177(-) (comEA) [Metabacillus sp. B2-18]
MSIIRKYKKWIILLVSLLVITILYYSFFNIQASSELVTANVEGAYIETSESYETSKVEEKISENSEAATIVIDVKGAVQQ
PGVYEISANARVHEIIEKAGGLSDHADEAAVNLASSLEDGMVVYIPLIGENKENPFQITNENKESSKKININIATSEELQ
TLSGIGPSKAEAIISYREENGKFKTIEGLLEVSGIGEKSLEKLKEEVTVN

Nucleotide


Download         Length: 633 bp        

>NTDB_id=633479 LPC09_RS17835 WP_231307912.1 3500545..3501177(-) (comEA) [Metabacillus sp. B2-18]
ATGAGTATCATCCGAAAATACAAAAAATGGATTATCTTATTGGTTAGTTTATTAGTTATAACCATCCTTTATTATTCTTT
TTTTAATATCCAAGCCTCATCTGAACTTGTGACAGCAAATGTTGAAGGTGCTTATATTGAAACAAGTGAAAGTTATGAAA
CAAGCAAGGTTGAGGAGAAAATAAGTGAAAATTCAGAAGCAGCTACCATCGTCATTGATGTAAAAGGGGCCGTTCAACAA
CCTGGAGTTTATGAAATTTCAGCAAATGCAAGAGTACATGAAATTATTGAAAAAGCTGGAGGATTGAGTGATCACGCTGA
TGAAGCGGCCGTAAACCTTGCCTCTTCATTAGAAGATGGAATGGTGGTTTATATTCCACTAATCGGTGAAAATAAAGAAA
ATCCATTTCAAATTACTAATGAAAATAAGGAAAGTTCTAAGAAAATCAATATTAACATAGCAACAAGTGAGGAGCTTCAA
ACACTATCAGGAATTGGACCTTCAAAGGCTGAAGCTATTATTTCTTATAGAGAAGAAAACGGTAAATTTAAAACGATTGA
GGGGTTATTAGAAGTATCAGGTATTGGAGAAAAGTCATTAGAAAAATTAAAAGAAGAAGTTACCGTTAATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(145-208)

SLBB domain (SLBB)

(71-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

48.113

100

0.486
  comEA Latilactobacillus sakei subsp. sakei 23K

37.288

100

0.419
  comEA/celA/cilE Streptococcus mitis SK321

38.249

100

0.395
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

37.788

100

0.39
  comEA Lactococcus lactis subsp. cremoris KW2

37.209

100

0.381