Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LQ331_RS03745 Genome accession   NZ_CP088240
Coordinates   813789..814931 (+) Length   380 a.a.
NCBI ID   WP_005408369.1    Uniprot ID   A0A854DW57
Organism   Stenotrophomonas maltophilia strain 2013-SM24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 808789..819931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ331_RS03715 (LQ331_03715) soxR 808903..809337 (+) 435 WP_005412484.1 redox-sensitive transcriptional activator SoxR -
  LQ331_RS03720 (LQ331_03720) - 809410..810036 (-) 627 Protein_711 short chain dehydrogenase -
  LQ331_RS03725 (LQ331_03725) - 810111..810986 (+) 876 WP_062606617.1 LysR family transcriptional regulator -
  LQ331_RS03730 (LQ331_03730) proC 811046..811867 (-) 822 WP_005408367.1 pyrroline-5-carboxylate reductase -
  LQ331_RS03735 (LQ331_03735) - 811886..812563 (-) 678 WP_062606619.1 YggS family pyridoxal phosphate-dependent enzyme -
  LQ331_RS03740 (LQ331_03740) pilT 812651..813688 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  LQ331_RS03745 (LQ331_03745) pilU 813789..814931 (+) 1143 WP_005408369.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LQ331_RS03750 (LQ331_03750) - 815139..816122 (+) 984 WP_062606621.1 aldo/keto reductase -
  LQ331_RS03755 (LQ331_03755) - 816170..817069 (-) 900 WP_062606848.1 LysR family transcriptional regulator -
  LQ331_RS03760 (LQ331_03760) - 817398..817610 (+) 213 WP_005408372.1 DUF4287 domain-containing protein -
  LQ331_RS03765 (LQ331_03765) - 817707..818156 (+) 450 WP_005408373.1 GNAT family N-acetyltransferase -
  LQ331_RS03770 (LQ331_03770) - 818249..818956 (+) 708 WP_024956077.1 YitT family protein -
  LQ331_RS03775 (LQ331_03775) - 819040..819585 (-) 546 WP_012479338.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42355.69 Da        Isoelectric Point: 6.7683

>NTDB_id=633395 LQ331_RS03745 WP_005408369.1 813789..814931(+) (pilU) [Stenotrophomonas maltophilia strain 2013-SM24]
MNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLG
MKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=633395 LQ331_RS03745 WP_005408369.1 813789..814931(+) (pilU) [Stenotrophomonas maltophilia strain 2013-SM24]
GTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGACCTGTTCAT
CACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGACCCCGCTCACGCCGCAGCAGA
GCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAATGCAACTTCGCCATC
GGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGCATCGA
GACGCGCATTCCGACGGTGGAAGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTCGGTGCCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAACTCGACCGGC
CACATCATCACCATCGAAGATCCGATTGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCAT
CGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTGCGTA
CCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCATGCCAACAACGCC
AACCAGGCGATGGACCGCATCGTCAATTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGACCTGTCGCTGAATCT
CAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTCGCGCAAGGTGGCGATGGAGATCCTGCTGGGCA
CGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGACTCGGTCCAGCTGGGC
ATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGTTACGCCGA
TTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCACGCACGCTGTCACAGGGCCTGGATGGCG
TGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A854DW57

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

96.579

0.655
  pilU Acinetobacter baylyi ADP1

63.585

93.947

0.597
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

93.421

0.403