Detailed information NTG0632285

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LQF05_RS01295
• Genome accession: NZ_CP088004
• Coordinates: 291401..292546 (+)
• Length: 381 a.a.
• NCBI ID: WP_019405534.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain SB-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 286401..297546

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LQF05_RS01275 (LQF05_01275)	-	288116..288706 (-)	591	WP_014598006.1	YggT family protein	-
LQF05_RS01280 (LQF05_01280)	proC	288716..289540 (-)	825	WP_108242417.1	pyrroline-5-carboxylate reductase	-
LQF05_RS01285 (LQF05_01285)	-	289551..290240 (-)	690	WP_108242416.1	YggS family pyridoxal phosphate-dependent enzyme	-
LQF05_RS01290 (LQF05_01290)	pilT	290307..291341 (+)	1035	WP_011914970.1	type IV pilus twitching motility protein PilT	Machinery gene
LQF05_RS01295 (LQF05_01295)	pilU	291401..292546 (+)	1146	WP_019405534.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LQF05_RS01300 (LQF05_01300)	-	292543..293376 (-)	834	WP_270067273.1	TIGR03915 family putative DNA repair protein	-
LQF05_RS01305 (LQF05_01305)	-	293376..294590 (-)	1215	WP_013984321.1	putative DNA modification/repair radical SAM protein	-
LQF05_RS01310 (LQF05_01310)	-	294726..295121 (+)	396	WP_011914966.1	NINE protein	-
LQF05_RS01315 (LQF05_01315)	-	295179..296453 (-)	1275	WP_095460261.1	dihydroorotase	-
LQF05_RS01320 (LQF05_01320)	-	296453..297469 (-)	1017	WP_236451263.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42566.88 Da
• Isoelectric Point: 6.8858

>NTDB_id=632285 LQF05_RS01295 WP_019405534.1 291401..292546(+) (pilU) [Stutzerimonas stutzeri strain SB-1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=632285 LQF05_RS01295 WP_019405534.1 291401..292546(+) (pilU) [Stutzerimonas stutzeri strain SB-1]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGTGCCCGCGGCATCGGCCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCGACGTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAATTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACCAGCTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGTACCCGCGAAACCATGGATTACG
CGGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAGGTCTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTTCCGACCCCGGACGGCAAGGGTCGTCGTGCGGTCATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACGTGCAGGGCGAGATCACCTACGAAGATGCCCTGCTGCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCAGCGTATCCCAGGGCCTGAGCCTGGAGGTCAGCGACG
AGGACCCGGGCCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.013	100	0.95
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.953	88.976	0.391