Detailed information NTG0632150

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: XYFPCFBP8416_RS05765
• Genome accession: NZ_CP087993
• Coordinates: 1297527..1298657 (+)
• Length: 376 a.a.
• NCBI ID: WP_154437071.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain CFBP8416
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1292527..1303657

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XYFPCFBP8416_RS05745 (XYFPCFBP8416_005695)	-	1292851..1293675 (+)	825	WP_004084943.1	glycosyltransferase family 2 protein	-
XYFPCFBP8416_RS05750 (XYFPCFBP8416_005700)	-	1294292..1295119 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
XYFPCFBP8416_RS05755 (XYFPCFBP8416_005705)	-	1295504..1295716 (-)	213	WP_021358439.1	hypothetical protein	-
XYFPCFBP8416_RS05760 (XYFPCFBP8416_005710)	pilT	1296338..1297372 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
XYFPCFBP8416_RS05765 (XYFPCFBP8416_005715)	pilU	1297527..1298657 (+)	1131	WP_154437071.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
XYFPCFBP8416_RS05770 (XYFPCFBP8416_005720)	-	1299326..1300294 (-)	969	WP_004084952.1	YafY family protein	-
XYFPCFBP8416_RS05775 (XYFPCFBP8416_005725)	-	1301077..1301886 (+)	810	WP_021358437.1	DUF481 domain-containing protein	-
XYFPCFBP8416_RS05780 (XYFPCFBP8416_005730)	hemC	1301940..1302857 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
XYFPCFBP8416_RS05785 (XYFPCFBP8416_005735)	-	1302865..1303623 (-)	759	WP_004084957.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42432.65 Da
• Isoelectric Point: 6.3881

>NTDB_id=632150 XYFPCFBP8416_RS05765 WP_154437071.1 1297527..1298657(+) (pilU) [Xylella fastidiosa subsp. multiplex strain CFBP8416]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHTNNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=632150 XYFPCFBP8416_RS05765 WP_154437071.1 1297527..1298657(+) (pilU) [Xylella fastidiosa subsp. multiplex strain CFBP8416]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATACCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	51.124	94.681	0.484
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.758	96.809	0.404