Detailed information NTG0632132

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LOK81_RS05735
• Genome accession: NZ_CP087992
• Coordinates: 1287524..1288654 (+)
• Length: 376 a.a.
• NCBI ID: WP_154437071.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain CFBP8433
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1282524..1293654

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LOK81_RS05715 (LOK81_05665)	-	1282848..1283672 (+)	825	WP_004084943.1	glycosyltransferase family 2 protein	-
LOK81_RS05720 (LOK81_05670)	-	1284289..1285116 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
LOK81_RS05725 (LOK81_05675)	-	1285501..1285713 (-)	213	WP_021358439.1	hypothetical protein	-
LOK81_RS05730 (LOK81_05680)	pilT	1286335..1287369 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
LOK81_RS05735 (LOK81_05685)	pilU	1287524..1288654 (+)	1131	WP_154437071.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LOK81_RS05740 (LOK81_05690)	-	1289323..1290291 (-)	969	WP_004084952.1	YafY family protein	-
LOK81_RS05745 (LOK81_05695)	-	1291074..1291883 (+)	810	WP_021358437.1	DUF481 domain-containing protein	-
LOK81_RS05750 (LOK81_05700)	hemC	1291937..1292854 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
LOK81_RS05755 (LOK81_05705)	-	1292862..1293620 (-)	759	WP_004084957.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42432.65 Da
• Isoelectric Point: 6.3881

>NTDB_id=632132 LOK81_RS05735 WP_154437071.1 1287524..1288654(+) (pilU) [Xylella fastidiosa subsp. multiplex strain CFBP8433]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHTNNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=632132 LOK81_RS05735 WP_154437071.1 1287524..1288654(+) (pilU) [Xylella fastidiosa subsp. multiplex strain CFBP8433]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATACCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	51.124	94.681	0.484
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.758	96.809	0.404