Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   SAGCMC97051_RS01550 Genome accession   NZ_AP020310
Coordinates   246002..246424 (+) Length   140 a.a.
NCBI ID   WP_001264244.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain GCMC97051     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 241002..251424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAGCMC97051_RS01505 (SAGCMC97051_02420) - 241402..242298 (+) 897 WP_001061853.1 sensor histidine kinase -
  SAGCMC97051_RS01510 (SAGCMC97051_02430) - 242292..242591 (+) 300 WP_000258211.1 STAS-like domain-containing protein -
  SAGCMC97051_RS01515 (SAGCMC97051_02440) - 242701..242901 (+) 201 WP_000076712.1 CsbD family protein -
  SAGCMC97051_RS01520 (SAGCMC97051_02450) - 242982..243233 (-) 252 WP_050196899.1 hypothetical protein -
  SAGCMC97051_RS01525 (SAGCMC97051_02460) - 243373..243519 (-) 147 WP_001030870.1 hypothetical protein -
  SAGCMC97051_RS01530 (SAGCMC97051_02470) - 243670..243879 (+) 210 WP_000424774.1 helix-turn-helix domain-containing protein -
  SAGCMC97051_RS01535 (SAGCMC97051_02480) - 243962..244786 (+) 825 WP_001125980.1 DUF3825 domain-containing protein -
  SAGCMC97051_RS01540 (SAGCMC97051_02490) - 245153..245698 (+) 546 WP_001870774.1 exotoxin beta-grasp domain-containing protein -
  SAGCMC97051_RS01545 (SAGCMC97051_02500) - 245706..245846 (+) 141 WP_161512414.1 hypothetical protein -
  SAGCMC97051_RS01550 (SAGCMC97051_02510) comGF/cglF 246002..246424 (+) 423 WP_001264244.1 competence type IV pilus minor pilin ComGF Machinery gene
  SAGCMC97051_RS01555 (SAGCMC97051_02520) comGG 246402..246773 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG Machinery gene
  SAGCMC97051_RS01560 (SAGCMC97051_02530) comGH 246888..247862 (+) 975 WP_001008574.1 class I SAM-dependent methyltransferase Machinery gene
  SAGCMC97051_RS01565 (SAGCMC97051_02540) - 247894..249087 (+) 1194 WP_000047534.1 acetate kinase -
  SAGCMC97051_RS01570 (SAGCMC97051_02550) - 249239..249445 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  SAGCMC97051_RS11560 - 249513..249641 (+) 129 WP_014959723.1 hypothetical protein -
  SAGCMC97051_RS01575 (SAGCMC97051_02560) - 249682..250137 (+) 456 WP_000905673.1 hypothetical protein -
  SAGCMC97051_RS01580 (SAGCMC97051_02570) - 250206..250871 (+) 666 WP_000008113.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 16234.79 Da        Isoelectric Point: 8.8708

>NTDB_id=63180 SAGCMC97051_RS01550 WP_001264244.1 246002..246424(+) (comGF/cglF) [Streptococcus agalactiae strain GCMC97051]
MRVLDTLLECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNKLYLRKQDKIVT
FGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide


Download         Length: 423 bp        

>NTDB_id=63180 SAGCMC97051_RS01550 WP_001264244.1 246002..246424(+) (comGF/cglF) [Streptococcus agalactiae strain GCMC97051]
GTGAGAGTTTTAGATACGCTTTTAGAATGTTTGGTAGCATTGGTTACAATCACAGGAGCTTTACTAGTTTATCAAGGACT
GACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTTCCAGTCAGTCTGAATGGGTGTTATTAACTCAGCAACTAA
ATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAGAACAAACTTTATTTACGTAAGCAAGATAAGATTGTAACC
TTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTATGATGGTCGAGGTTATCAACCAATGGTTTATGGGTTAGA
CAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTGTTTTTTATTTTAAGGACGGGTTAAAAAGGACATTTTACT
ATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(36-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

41.791

95.714

0.4
  comGF/cglF Streptococcus pneumoniae D39

41.791

95.714

0.4
  comGF/cglF Streptococcus pneumoniae R6

41.791

95.714

0.4
  comGF/cglF Streptococcus pneumoniae TIGR4

41.791

95.714

0.4
  comGF Streptococcus mutans UA159

45.161

88.571

0.4
  comGF Streptococcus mutans UA140

45.161

88.571

0.4
  comGF/cglF Streptococcus mitis NCTC 12261

42.636

92.143

0.393
  comGF/cglF Streptococcus mitis SK321

42.636

92.143

0.393
  comGF Lactococcus lactis subsp. cremoris KW2

39.85

95

0.379

Multiple sequence alignment