Detailed information NTG0063179

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: U471_RS11880
• Genome accession: NC_022653
• Coordinates: 2507095..2507709 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus amyloliquefaciens CC178
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2502095..2512709

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U471_RS11855 (U471_24670)	rpsT	2502398..2502664 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
U471_RS11860 (U471_24680)	holA	2502681..2503722 (-)	1042	Protein_2347	DNA polymerase III subunit delta	-
U471_RS19785	-	2503762..2503913 (-)	152	Protein_2348	hypothetical protein	-
U471_RS19410 (U471_24690)	-	2503954..2504088 (+)	135	WP_003152870.1	YqzM family protein	-
U471_RS11870 (U471_24700)	comEC	2504107..2506458 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
U471_RS11875 (U471_24710)	-	2506459..2507028 (-)	570	WP_003152868.1	ComE operon protein 2	-
U471_RS11880 (U471_24720)	comEA	2507095..2507709 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
U471_RS11885 (U471_24730)	comER	2507768..2508589 (+)	822	WP_012118027.1	late competence protein ComER	-
U471_RS11890 (U471_24740)	-	2508658..2509395 (-)	738	WP_012118028.1	class I SAM-dependent DNA methyltransferase	-
U471_RS11895 (U471_24750)	rsfS	2509392..2509748 (-)	357	WP_007408260.1	ribosome silencing factor	-
U471_RS11900 (U471_24760)	yqeK	2509765..2510325 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
U471_RS11905 (U471_24770)	-	2510315..2510884 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
U471_RS11910 (U471_24780)	yhbY	2510895..2511185 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
U471_RS11915 (U471_24790)	aroE	2511179..2512021 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=63179 U471_RS11880 WP_012118026.1 2507095..2507709(-) (comEA) [Bacillus amyloliquefaciens CC178]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=63179 U471_RS11880 WP_012118026.1 2507095..2507709(-) (comEA) [Bacillus amyloliquefaciens CC178]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363