Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   N5B54_RS03825 Genome accession   NZ_CP104592
Coordinates   782391..783884 (+) Length   497 a.a.
NCBI ID   WP_087597520.1    Uniprot ID   -
Organism   Escherichia coli strain EC5931     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 777391..788884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5B54_RS03800 (N5B54_03800) - 777837..778534 (+) 698 WP_223216367.1 IS1 family transposase -
  N5B54_RS03805 (N5B54_03805) pppA 778546..778848 (+) 303 Protein_747 prepilin peptidase PppA -
  N5B54_RS03810 (N5B54_03810) gspS2 778914..779324 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  N5B54_RS03815 (N5B54_03815) gspC 779342..780301 (+) 960 WP_000135091.1 type II secretion system protein GspC -
  N5B54_RS03820 (N5B54_03820) gspD 780331..782391 (+) 2061 WP_000498830.1 type II secretion system secretin GspD -
  N5B54_RS03825 (N5B54_03825) pilB/pilB1 782391..783884 (+) 1494 WP_087597520.1 type II secretion system ATPase GspE Machinery gene
  N5B54_RS03830 (N5B54_03830) gspF 783884..785107 (+) 1224 WP_001173433.1 type II secretion system inner membrane protein GspF -
  N5B54_RS03835 (N5B54_03835) gspG 785124..785579 (+) 456 WP_149851811.1 type II secretion system major pseudopilin GspG -
  N5B54_RS03840 (N5B54_03840) gspH 785583..786146 (+) 564 WP_024230000.1 type II secretion system minor pseudopilin GspH -
  N5B54_RS03845 (N5B54_03845) gspI 786143..786514 (+) 372 WP_000820090.1 type II secretion system minor pseudopilin GspI -
  N5B54_RS03850 (N5B54_03850) gspJ 786511..787116 (+) 606 WP_024229999.1 type II secretion system minor pseudopilin GspJ -
  N5B54_RS03855 (N5B54_03855) gspK 787113..788090 (+) 978 WP_000633199.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55208.39 Da        Isoelectric Point: 6.1641

>NTDB_id=631455 N5B54_RS03825 WP_087597520.1 782391..783884(+) (pilB/pilB1) [Escherichia coli strain EC5931]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRKIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCTDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=631455 N5B54_RS03825 WP_087597520.1 782391..783884(+) (pilB/pilB1) [Escherichia coli strain EC5931]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTTTCTGTTTTACAGGAGCTACAGCGGGGGCTGA
ATGCGCCCTTTACACTGCGGAAAATCGACGAGGCTGAATTTGAACAGCGACTGAATGCGGTCTGGCAGCGGGACTCCTCC
GAGGCTCGCCAGTTGATGGAAGATCTCGGCTCCGCCGAGGACTTTTTTACCCTCGCAGAAGAGTTACCGGAAACCGAGGA
TTTGCTGGAAAGTGACGATGATGCGCCGATCATCAAACTGATCAATGCTATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCTACCATGCCTTCAGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCACTCGCGCAACATTCTCACAGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTGCTTTCGACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTCGAGCCTTTCCTGC
TCTCTTCCAGCCTGACGGGCGTGATGGCGCAGCGGCTGGTCCGCACGCTGTGCACCGACTGCCGCCAGCCCGCGCCTGCC
ACTGATGAAGAAAAACGCCTGCTGGGGATTACCGACGCGCGCACCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTGCTGCGGGTCACGATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362