Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   N5B54_RS03605 Genome accession   NZ_CP104592
Coordinates   737370..738029 (-) Length   219 a.a.
NCBI ID   WP_001221490.1    Uniprot ID   -
Organism   Escherichia coli strain EC5931     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 732370..743029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5B54_RS03575 (N5B54_03575) ygiN 733271..733585 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  N5B54_RS03580 (N5B54_03580) mdaB 733616..734197 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  N5B54_RS03585 (N5B54_03585) - 734447..734926 (+) 480 WP_000065331.1 Hcp family type VI secretion system effector -
  N5B54_RS03590 (N5B54_03590) - 734929..735639 (+) 711 WP_000834022.1 hypothetical protein -
  N5B54_RS03595 (N5B54_03595) ygiZ 735646..735978 (+) 333 WP_000914696.1 DUF2645 family protein -
  N5B54_RS03600 (N5B54_03600) qseC 736024..737373 (-) 1350 WP_000673389.1 quorum sensing histidine kinase QseC -
  N5B54_RS03605 (N5B54_03605) ciaR 737370..738029 (-) 660 WP_001221490.1 quorum sensing response regulator transcription factor QseB Regulator
  N5B54_RS03610 (N5B54_03610) ygiW 738181..738573 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  N5B54_RS03615 (N5B54_03615) ygiV 738626..739108 (+) 483 WP_000183484.1 GyrI-like domain-containing protein -
  N5B54_RS03620 (N5B54_03620) ygiS 739217..740824 (+) 1608 WP_000288656.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24663.65 Da        Isoelectric Point: 6.8808

>NTDB_id=631453 N5B54_RS03605 WP_001221490.1 737370..738029(-) (ciaR) [Escherichia coli strain EC5931]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELLRGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=631453 N5B54_RS03605 WP_001221490.1 737370..738029(-) (ciaR) [Escherichia coli strain EC5931]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCTCCGCGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365
  covR Lactococcus lactis subsp. lactis strain DGCC12653

34.802

100

0.361