Detailed information NTG0631408

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N5878_RS23065
• Genome accession: NZ_CP104590
• Coordinates: 4955143..4956291 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain WTJH36
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4950143..4961291

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5878_RS23040 (N5878_23040)	-	4951294..4951590 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
N5878_RS23045 (N5878_23045)	-	4951587..4952180 (-)	594	WP_003084544.1	YggT family protein	-
N5878_RS23050 (N5878_23050)	proC	4952191..4953012 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
N5878_RS23055 (N5878_23055)	-	4953024..4953716 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
N5878_RS23060 (N5878_23060)	pilT	4953931..4954965 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
N5878_RS23065 (N5878_23065)	pilU	4955143..4956291 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
N5878_RS23070 (N5878_23070)	czcD	4956299..4957198 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
N5878_RS23075 (N5878_23075)	-	4957353..4957757 (+)	405	WP_003084560.1	NINE protein	-
N5878_RS23080 (N5878_23080)	-	4958000..4959373 (+)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
N5878_RS23085 (N5878_23085)	-	4959370..4960554 (+)	1185	WP_016852373.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=631408 N5878_RS23065 WP_003100959.1 4955143..4956291(+) (pilU) [Pseudomonas aeruginosa strain WTJH36]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=631408 N5878_RS23065 WP_003100959.1 4955143..4956291(+) (pilU) [Pseudomonas aeruginosa strain WTJH36]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382