Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LPW13_RS14750 Genome accession   NZ_CP087715
Coordinates   3579753..3580829 (-) Length   358 a.a.
NCBI ID   WP_230436537.1    Uniprot ID   -
Organism   Microbulbifer celer strain KCTC 12973     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3574753..3585829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPW13_RS14725 (LPW13_14725) - 3575268..3575792 (-) 525 WP_230439207.1 shikimate kinase -
  LPW13_RS14730 (LPW13_14730) pilQ 3575792..3578020 (-) 2229 WP_230436530.1 type IV pilus secretin PilQ -
  LPW13_RS14735 (LPW13_14735) - 3578023..3578550 (-) 528 WP_230436532.1 pilus assembly protein PilP -
  LPW13_RS14740 (LPW13_14740) - 3578547..3579167 (-) 621 WP_230436534.1 type 4a pilus biogenesis protein PilO -
  LPW13_RS14745 (LPW13_14745) - 3579170..3579751 (-) 582 WP_230436536.1 PilN domain-containing protein -
  LPW13_RS14750 (LPW13_14750) pilM 3579753..3580829 (-) 1077 WP_230436537.1 pilus assembly protein PilM Machinery gene
  LPW13_RS14755 (LPW13_14755) - 3580997..3583690 (+) 2694 WP_230436539.1 penicillin-binding protein 1A -
  LPW13_RS14760 (LPW13_14760) - 3583805..3584290 (-) 486 WP_230436541.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38794.97 Da        Isoelectric Point: 4.3560

>NTDB_id=631180 LPW13_RS14750 WP_230436537.1 3579753..3580829(-) (pilM) [Microbulbifer celer strain KCTC 12973]
MGMLPFLNKGPKALLGLDISSTSVKLLELSRNGDKYRVESYATEPLPPNAVVDKNINDVAATAEAIRKVVRRSKTRLRQA
AVAVSGSAVITKTLDMPADLSDEALEAQIALEADQYIPYPLDEVNLDFEVQGVSERGEGQAEVLLAACRSENIEQRVSAL
GEADLQAGVVDVEAYAIERAYTLMDSQFPAQEQLVVAVIDIGATMSALSVMVDGRTVYTREQLFGGRQLTDEIQRRYGLS
AEEATLAKRQGGSGLPDDYYPEVLEPFRDAVVQQVTRSLQFFYSSTSYSDVDYILLGGGVAAMDGLAELVGQKLNKPTLV
ANPFRDMSVASRVSRQALAAEAPSLMIAAGLAMREKDY

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=631180 LPW13_RS14750 WP_230436537.1 3579753..3580829(-) (pilM) [Microbulbifer celer strain KCTC 12973]
ATGGGGATGCTCCCTTTTCTCAATAAGGGGCCTAAGGCACTGCTTGGGCTCGATATTAGCTCCACCTCGGTAAAGTTGCT
TGAACTGAGTCGCAATGGCGACAAATACCGCGTGGAAAGCTATGCAACAGAGCCTCTGCCTCCGAATGCGGTAGTGGACA
AAAATATCAACGATGTCGCTGCCACCGCGGAAGCCATCCGCAAGGTGGTGCGCCGCTCCAAGACCCGTTTGCGCCAGGCA
GCTGTGGCCGTTTCCGGTTCAGCAGTCATCACCAAGACCCTGGATATGCCCGCGGACCTGTCCGACGAGGCGCTGGAAGC
GCAGATTGCCCTGGAGGCCGATCAGTACATTCCGTACCCGCTGGACGAGGTGAACCTCGACTTTGAGGTGCAGGGAGTGT
CCGAGCGGGGTGAGGGGCAGGCGGAAGTGTTGCTGGCCGCGTGTCGCAGCGAGAACATCGAACAGCGAGTTTCCGCGCTG
GGTGAGGCGGATCTGCAGGCCGGTGTCGTCGACGTTGAGGCCTATGCGATCGAGCGCGCCTATACCCTGATGGACAGCCA
GTTTCCGGCTCAGGAGCAGCTGGTGGTCGCGGTCATCGATATCGGTGCCACCATGAGTGCACTGTCGGTCATGGTGGATG
GTCGCACCGTGTATACTCGCGAGCAGCTGTTCGGCGGGCGTCAGCTCACCGATGAAATCCAGCGCCGTTACGGTCTGTCT
GCGGAAGAGGCGACACTGGCCAAGCGCCAGGGTGGCAGCGGACTGCCGGATGACTACTATCCGGAAGTGCTGGAGCCCTT
CCGCGATGCGGTGGTGCAGCAGGTCACCCGCTCCCTGCAATTTTTCTACTCATCGACCTCCTACAGCGATGTGGACTACA
TCCTGCTCGGTGGTGGCGTCGCCGCCATGGACGGGCTGGCAGAGCTCGTGGGGCAAAAGCTCAATAAGCCGACCCTGGTG
GCCAATCCGTTCCGGGACATGAGTGTCGCCTCGCGGGTGAGTCGCCAGGCACTGGCCGCGGAGGCGCCGTCGCTGATGAT
TGCCGCCGGCCTCGCCATGCGGGAGAAGGATTACTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.549

99.162

0.511
  comM Acinetobacter nosocomialis M2

51.549

99.162

0.511
  comM Acinetobacter baylyi ADP1

46.761

99.162

0.464
  pilM Legionella pneumophila strain ERS1305867

43.22

98.883

0.427