Detailed information NTG0631166

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N5877_RS12630
• Genome accession: NZ_CP104586
• Coordinates: 2727747..2728895 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain WTJH6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2722747..2733895

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5877_RS12610 (N5877_12610)	-	2723485..2724669 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
N5877_RS12615 (N5877_12615)	-	2724666..2726039 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
N5877_RS12620 (N5877_12620)	-	2726282..2726686 (-)	405	WP_003084560.1	NINE protein	-
N5877_RS12625 (N5877_12625)	czcD	2726840..2727739 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
N5877_RS12630 (N5877_12630)	pilU	2727747..2728895 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
N5877_RS12635 (N5877_12635)	pilT	2729073..2730107 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
N5877_RS12640 (N5877_12640)	-	2730322..2731014 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
N5877_RS12645 (N5877_12645)	proC	2731026..2731847 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
N5877_RS12650 (N5877_12650)	-	2731858..2732451 (+)	594	WP_003084544.1	YggT family protein	-
N5877_RS12655 (N5877_12655)	-	2732448..2732744 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=631166 N5877_RS12630 WP_003100959.1 2727747..2728895(-) (pilU) [Pseudomonas aeruginosa strain WTJH6]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=631166 N5877_RS12630 WP_003100959.1 2727747..2728895(-) (pilU) [Pseudomonas aeruginosa strain WTJH6]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382