Detailed information NTG0063108

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: EcoO8H8_RS09265
• Genome accession: NZ_AP019856
• Coordinates: 1957297..1958307 (-)
• Length: 336 a.a.
• NCBI ID: WP_000568514.1
• Uniprot ID: -
• Organism: Escherichia coli O8:H8 strain 16F5M1D1
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1952297..1963307

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EcoO8H8_RS09245 (Eco16F5M1D1_1835)	mepM	1953264..1954586 (-)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-
EcoO8H8_RS09250 (Eco16F5M1D1_1836)	znuA	1954602..1955534 (-)	933	WP_096307053.1	zinc ABC transporter substrate-binding protein ZnuA	-
EcoO8H8_RS09255 (Eco16F5M1D1_1837)	znuC	1955613..1956368 (+)	756	WP_000202980.1	zinc ABC transporter ATP-binding protein ZnuC	-
EcoO8H8_RS09260 (Eco16F5M1D1_1838)	znuB	1956365..1957150 (+)	786	WP_000571479.1	zinc ABC transporter permease subunit ZnuB	-
EcoO8H8_RS09265 (Eco16F5M1D1_1839)	ruvB	1957297..1958307 (-)	1011	WP_000568514.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
EcoO8H8_RS09270 (Eco16F5M1D1_1840)	ruvA	1958316..1958927 (-)	612	WP_000580327.1	Holliday junction branch migration protein RuvA	-
EcoO8H8_RS09275	yobI	1959066..1959131 (-)	66	WP_072094247.1	stress response small protein YobI	-
EcoO8H8_RS09280 (Eco16F5M1D1_1841)	yebB	1959203..1959805 (+)	603	WP_058101573.1	YebB family permuted papain-like enzyme	-
EcoO8H8_RS09285 (Eco16F5M1D1_1842)	ruvC	1959807..1960328 (-)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
EcoO8H8_RS09290 (Eco16F5M1D1_1843)	yebC	1960363..1961103 (-)	741	WP_000907248.1	YebC/PmpR family DNA-binding transcriptional regulator	-
EcoO8H8_RS09295 (Eco16F5M1D1_1844)	nudB	1961132..1961584 (-)	453	WP_001300367.1	dihydroneopterin triphosphate diphosphatase	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37187.80 Da
• Isoelectric Point: 4.7818

>NTDB_id=63108 EcoO8H8_RS09265 WP_000568514.1 1957297..1958307(-) (ruvB) [Escherichia coli O8:H8 strain 16F5M1D1]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEIARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=63108 EcoO8H8_RS09265 WP_000568514.1 1957297..1958307(-) (ruvB) [Escherichia coli O8:H8 strain 16F5M1D1]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAATTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAAGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.79	97.917	0.595
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.56	96.131	0.515
ruvB	Helicobacter pylori 26695	52.351	94.94	0.497