Detailed information NTG0631029

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N5876_RS02240
• Genome accession: NZ_CP104584
• Coordinates: 488800..489948 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain WTJH2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 483800..494948

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5876_RS02215 (N5876_02215)	-	484951..485247 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
N5876_RS02220 (N5876_02220)	-	485244..485837 (-)	594	WP_003084544.1	YggT family protein	-
N5876_RS02225 (N5876_02225)	proC	485848..486669 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
N5876_RS02230 (N5876_02230)	-	486681..487373 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
N5876_RS02235 (N5876_02235)	pilT	487588..488622 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
N5876_RS02240 (N5876_02240)	pilU	488800..489948 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
N5876_RS02245 (N5876_02245)	czcD	489956..490855 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
N5876_RS02250 (N5876_02250)	-	491010..491414 (+)	405	WP_003084560.1	NINE protein	-
N5876_RS02255 (N5876_02255)	-	491657..493030 (+)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
N5876_RS02260 (N5876_02260)	-	493027..494211 (+)	1185	WP_016852373.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=631029 N5876_RS02240 WP_003100959.1 488800..489948(+) (pilU) [Pseudomonas aeruginosa strain WTJH2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=631029 N5876_RS02240 WP_003100959.1 488800..489948(+) (pilU) [Pseudomonas aeruginosa strain WTJH2]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382