Detailed information NTG0630743

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LO759_RS02085
• Genome accession: NZ_CP087675
• Coordinates: 453549..454697 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain P93127
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 448549..459697

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LO759_RS02060 (LO759_02060)	-	449700..449996 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
LO759_RS02065 (LO759_02065)	-	449993..450586 (-)	594	WP_003084544.1	YggT family protein	-
LO759_RS02070 (LO759_02070)	proC	450597..451418 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LO759_RS02075 (LO759_02075)	-	451430..452122 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
LO759_RS02080 (LO759_02080)	pilT	452337..453371 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LO759_RS02085 (LO759_02085)	pilU	453549..454697 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
LO759_RS02090 (LO759_02090)	czcD	454705..455604 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LO759_RS02095 (LO759_02095)	-	455759..456163 (+)	405	WP_003084560.1	NINE protein	-
LO759_RS02100 (LO759_02100)	-	456406..457779 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
LO759_RS02105 (LO759_02105)	-	457776..458960 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=630743 LO759_RS02085 WP_003110016.1 453549..454697(+) (pilU) [Pseudomonas aeruginosa strain P93127]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=630743 LO759_RS02085 WP_003110016.1 453549..454697(+) (pilU) [Pseudomonas aeruginosa strain P93127]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382