Detailed information NTG0630708

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LO758_RS02085
• Genome accession: NZ_CP087674
• Coordinates: 453543..454691 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain P4970C
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 448543..459691

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LO758_RS02060 (LO758_02060)	-	449694..449990 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
LO758_RS02065 (LO758_02065)	-	449987..450580 (-)	594	WP_003084544.1	YggT family protein	-
LO758_RS02070 (LO758_02070)	proC	450591..451412 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LO758_RS02075 (LO758_02075)	-	451424..452116 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
LO758_RS02080 (LO758_02080)	pilT	452331..453365 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LO758_RS02085 (LO758_02085)	pilU	453543..454691 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
LO758_RS02090 (LO758_02090)	czcD	454699..455598 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LO758_RS02095 (LO758_02095)	-	455753..456157 (+)	405	WP_003084560.1	NINE protein	-
LO758_RS02100 (LO758_02100)	-	456400..457773 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
LO758_RS02105 (LO758_02105)	-	457770..458954 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=630708 LO758_RS02085 WP_003110016.1 453543..454691(+) (pilU) [Pseudomonas aeruginosa strain P4970C]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=630708 LO758_RS02085 WP_003110016.1 453543..454691(+) (pilU) [Pseudomonas aeruginosa strain P4970C]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382