Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   N5O72_RS03605 Genome accession   NZ_CP104536
Coordinates   731767..732426 (-) Length   219 a.a.
NCBI ID   WP_001221491.1    Uniprot ID   -
Organism   Escherichia coli strain E3     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 726767..737426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O72_RS03580 (N5O72_03580) - 726971..727723 (-) 753 WP_000619462.1 GntR family transcriptional regulator -
  N5O72_RS03585 (N5O72_03585) - 728010..729341 (+) 1332 Protein_702 MFS transporter -
  N5O72_RS03590 (N5O72_03590) ygiN 729385..729699 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  N5O72_RS03595 (N5O72_03595) mdaB 729730..730311 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  N5O72_RS03600 (N5O72_03600) qseC 730421..731770 (-) 1350 WP_000673406.1 quorum sensing histidine kinase QseC -
  N5O72_RS03605 (N5O72_03605) ciaR 731767..732426 (-) 660 WP_001221491.1 quorum sensing response regulator transcription factor QseB Regulator
  N5O72_RS03610 (N5O72_03610) ygiW 732578..732970 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  N5O72_RS03615 (N5O72_03615) ygiV 733023..733505 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  N5O72_RS03620 (N5O72_03620) mqsR 733710..734006 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  N5O72_RS03625 (N5O72_03625) mqsA 734008..734403 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  N5O72_RS03630 (N5O72_03630) ygiS 734536..736143 (+) 1608 WP_001701096.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6540

>NTDB_id=630570 N5O72_RS03605 WP_001221491.1 731767..732426(-) (ciaR) [Escherichia coli strain E3]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPDKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=630570 N5O72_RS03605 WP_001221491.1 731767..732426(-) (ciaR) [Escherichia coli strain E3]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGACAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365
  micA Streptococcus pneumoniae Cp1015

34.498

100

0.361