Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   N5O75_RS03985 Genome accession   NZ_CP104500
Coordinates   820215..821708 (+) Length   497 a.a.
NCBI ID   WP_038976828.1    Uniprot ID   -
Organism   Escherichia coli strain E9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 815215..826708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O75_RS03965 (N5O75_03965) pilD 815863..816672 (+) 810 WP_001365924.1 prepilin peptidase PppA Machinery gene
  N5O75_RS03970 (N5O75_03970) gspS2 816738..817148 (+) 411 WP_001298744.1 type II secretion system pilot lipoprotein GspS-beta -
  N5O75_RS03975 (N5O75_03975) gspC 817166..818125 (+) 960 WP_261306854.1 type II secretion system protein GspC -
  N5O75_RS03980 (N5O75_03980) gspD 818155..820215 (+) 2061 WP_038976827.1 type II secretion system secretin GspD -
  N5O75_RS03985 (N5O75_03985) pilB/pilB1 820215..821708 (+) 1494 WP_038976828.1 type II secretion system ATPase GspE Machinery gene
  N5O75_RS03990 (N5O75_03990) gspF 821708..822931 (+) 1224 WP_038976829.1 type II secretion system inner membrane protein GspF -
  N5O75_RS03995 (N5O75_03995) gspG 822948..823403 (+) 456 WP_021534529.1 type II secretion system major pseudopilin GspG -
  N5O75_RS04000 (N5O75_04000) gspH 823407..823970 (+) 564 WP_001115138.1 type II secretion system minor pseudopilin GspH -
  N5O75_RS04005 (N5O75_04005) gspI 823967..824338 (+) 372 WP_038976830.1 type II secretion system minor pseudopilin GspI -
  N5O75_RS04010 (N5O75_04010) gspJ 824335..824940 (+) 606 WP_038976831.1 type II secretion system minor pseudopilin GspJ -
  N5O75_RS04015 (N5O75_04015) gspK 824937..825914 (+) 978 WP_038976832.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55259.44 Da        Isoelectric Point: 6.0390

>NTDB_id=629903 N5O75_RS03985 WP_038976828.1 820215..821708(+) (pilB/pilB1) [Escherichia coli strain E9]
MVPVAQETTANTVRLPYSFSRRFNLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=629903 N5O75_RS03985 WP_038976828.1 820215..821708(+) (pilB/pilB1) [Escherichia coli strain E9]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAACCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGTTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACTCTCGCTGAAGAACTGCCGGAAACTGAAGA
TCTGCTGGAAAGTGACGACGATGCACCGATCATCAAACTGATTAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGC
CCTGGGCGCAAACTGGCCTCGCTGCTGGTCTCACGAATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGCATTGCGCTGCTGCTGGGAGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACA
GTGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGACCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATTGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGGGTAGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGCCCGCACCAGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAATGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Legionella pneumophila strain ERS1305867

41.598

98.189

0.408
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.615

79.879

0.388
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362