Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   N4P55_RS04665 Genome accession   NZ_CP104408
Coordinates   1077460..1078953 (-) Length   497 a.a.
NCBI ID   WP_064450721.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain Ant01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072460..1083953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4P55_RS04630 (N4P55_04640) - 1073434..1073877 (+) 444 WP_128588501.1 hypothetical protein -
  N4P55_RS04635 (N4P55_04645) - 1073879..1074490 (+) 612 WP_262667033.1 superoxide dismutase -
  N4P55_RS04640 (N4P55_04650) - 1074522..1075415 (+) 894 WP_262667034.1 ZIP family metal transporter -
  N4P55_RS04645 (N4P55_04655) - 1075453..1075725 (-) 273 WP_049709983.1 HPr family phosphocarrier protein -
  N4P55_RS04650 (N4P55_04660) rapZ 1075742..1076599 (-) 858 WP_053254418.1 RNase adapter RapZ -
  N4P55_RS04655 (N4P55_04665) ptsN 1076602..1077066 (-) 465 WP_262667035.1 PTS IIA-like nitrogen regulatory protein PtsN -
  N4P55_RS04660 (N4P55_04670) hpf 1077079..1077384 (-) 306 WP_017138237.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  N4P55_RS04665 (N4P55_04675) rpoN/rpoN1 1077460..1078953 (-) 1494 WP_064450721.1 RNA polymerase factor sigma-54 Machinery gene
  N4P55_RS04670 (N4P55_04680) lptB 1079138..1079863 (-) 726 WP_053254421.1 LPS export ABC transporter ATP-binding protein -
  N4P55_RS04675 (N4P55_04685) lptA 1079863..1080438 (-) 576 WP_064450722.1 lipopolysaccharide transport periplasmic protein LptA -
  N4P55_RS04680 (N4P55_04690) lptC 1080425..1080997 (-) 573 WP_064450723.1 LPS export ABC transporter periplasmic protein LptC -
  N4P55_RS04685 (N4P55_04695) - 1081006..1081530 (-) 525 WP_064450724.1 KdsC family phosphatase -
  N4P55_RS04690 (N4P55_04700) - 1081531..1082505 (-) 975 WP_053254425.1 KpsF/GutQ family sugar-phosphate isomerase -
  N4P55_RS04695 (N4P55_04705) - 1082804..1083613 (+) 810 WP_262667036.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56624.74 Da        Isoelectric Point: 4.3925

>NTDB_id=629299 N4P55_RS04665 WP_064450721.1 1077460..1078953(-) (rpoN/rpoN1) [Pseudomonas fluorescens strain Ant01]
MKPSLVLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEEGDDFDNADPLADNIEQKPNSDVQEPSYQ
ETAPTVDNLEEGEWNERIPNELPVDTAWEDVYQTSASSLPSNDDDEWDFTTRTSVGESLQSHLLWQLNLAPMSDTDRLIA
VTLIDCINNQGYLDETLEEILEAFDPELDIELDEIEAVLHRIQQFEPAGIGARTLSECLLLQLRQLPAKTPWLAEAQRLV
TDYIDLLGSRDYSQLMRRMKLKEDDLRQVIELVQSLNPRPGSQIESSEAEYVVPDVIVRKDNERWLVELNQESVPRLRVN
PQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLEYGDEAMKPLVLHDIAEAVGMHEST
ISRVTTQKFMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLM

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=629299 N4P55_RS04665 WP_064450721.1 1077460..1078953(-) (rpoN/rpoN1) [Pseudomonas fluorescens strain Ant01]
ATGAAACCATCGCTAGTCCTGAGAATGGGCCAGCAGCTGACGATGACACCGCAGCTGCAACAGGCCATCCGCCTGCTCCA
ATTGTCGACCCTGGACCTGCAACAGGAAATCCAGGAGGCCCTGGAGTCCAATCCGATGCTCGAACGCCAGGAAGAAGGCG
ACGACTTCGATAATGCAGACCCGCTGGCCGACAACATCGAGCAAAAACCCAATTCCGACGTGCAGGAACCTTCCTATCAG
GAAACCGCCCCTACGGTGGACAACCTTGAGGAAGGCGAATGGAACGAACGCATTCCCAACGAGCTTCCGGTAGACACCGC
CTGGGAAGACGTTTACCAGACCAGCGCCAGCAGCCTGCCCAGCAATGATGATGACGAGTGGGATTTCACCACCCGCACCT
CCGTCGGCGAGAGCTTGCAGAGCCATTTGCTGTGGCAATTGAACCTGGCGCCGATGTCCGACACCGATCGCCTGATCGCC
GTGACCCTGATCGACTGCATCAATAACCAGGGTTATCTGGACGAGACTCTCGAAGAGATCCTTGAGGCTTTCGATCCGGA
ACTGGACATCGAACTGGACGAAATCGAAGCCGTCCTGCACCGCATCCAGCAGTTCGAGCCCGCCGGTATCGGCGCGCGCA
CGCTGAGCGAGTGCCTGTTGCTGCAACTGCGCCAACTGCCTGCCAAGACCCCGTGGCTCGCCGAAGCCCAGCGCCTGGTC
ACCGACTACATCGACCTGCTGGGCAGCCGCGACTACAGCCAGTTGATGCGCCGTATGAAGCTCAAGGAAGACGACCTGCG
CCAGGTCATCGAGCTGGTGCAGAGCCTTAACCCTCGCCCGGGCTCGCAGATCGAGTCCAGCGAAGCCGAATATGTCGTTC
CCGATGTGATCGTGCGCAAAGACAACGAACGTTGGCTGGTGGAGCTGAACCAGGAGTCGGTGCCTCGTCTGCGGGTCAAC
CCGCAATACGCAGGTTTTGTGCGCCGTGCCGATACCAGCGCCGATAACACCTTCATGCGCAACCAGTTGCAGGAAGCGCG
CTGGTTCATCAAGAGCCTGCAAAGCCGCAACGAAACCCTGATGAAAGTGGCTACTCAGATCGTCGAGCATCAACGCGGCT
TCCTGGAATACGGCGACGAGGCCATGAAACCCCTGGTCCTGCACGATATCGCCGAAGCGGTAGGCATGCATGAATCGACA
ATTTCCAGGGTGACCACACAGAAATTCATGCATACCCCACGGGGTATTTATGAGCTGAAATACTTTTTCTCCAGCCACGT
AAGCACCTCCGAAGGCGGCGAATGCTCGTCCACGGCGATCCGCGCGATCATCAAAAAACTGGTTGCCGCGGAAAATCAGA
AAAAGCCGTTGAGTGACAGCAAGATCGCTGGTTTACTGGAGGCACAAGGCATTCAGGTCGCCCGTCGAACCGTCGCCAAG
TACCGCGAGTCCCTCGGGATCGCGCCTTCCAGCGAGCGTAAGCGTTTGATGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-323)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

43.936

100

0.445