Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   N4R43_RS01635 Genome accession   NZ_CP104388
Coordinates   269991..271064 (-) Length   357 a.a.
NCBI ID   WP_081041295.1    Uniprot ID   -
Organism   Lactococcus lactis strain LJL7m20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 264991..276064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4R43_RS01595 (N4R43_01595) - 265187..265309 (-) 123 WP_023164646.1 hypothetical protein -
  N4R43_RS01600 (N4R43_01600) - 265306..265488 (-) 183 WP_003130605.1 hypothetical protein -
  N4R43_RS01605 (N4R43_01605) - 265504..266220 (-) 717 WP_317141374.1 phage antirepressor KilAC domain-containing protein -
  N4R43_RS01610 (N4R43_01610) - 266266..266487 (-) 222 WP_317141375.1 helix-turn-helix transcriptional regulator -
  N4R43_RS01615 (N4R43_01615) - 266643..267065 (+) 423 WP_317141376.1 helix-turn-helix transcriptional regulator -
  N4R43_RS01620 (N4R43_01620) - 267076..267660 (+) 585 WP_288625240.1 hypothetical protein -
  N4R43_RS01625 (N4R43_01625) - 267715..268254 (+) 540 WP_023164651.1 PH domain-containing protein -
  N4R43_RS01630 (N4R43_01630) - 268380..269838 (+) 1459 Protein_313 recombinase family protein -
  N4R43_RS01635 (N4R43_01635) comGB 269991..271064 (-) 1074 WP_081041295.1 competence type IV pilus assembly protein ComGB Machinery gene
  N4R43_RS01640 (N4R43_01640) comGA 270958..271896 (-) 939 WP_031561106.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40339.52 Da        Isoelectric Point: 10.3001

>NTDB_id=629202 N4R43_RS01635 WP_081041295.1 269991..271064(-) (comGB) [Lactococcus lactis strain LJL7m20]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=629202 N4R43_RS01635 WP_081041295.1 269991..271064(-) (comGB) [Lactococcus lactis strain LJL7m20]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAGGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

83.284

95.518

0.796
  comGB Streptococcus gordonii str. Challis substr. CH1

53.959

95.518

0.515
  comGB Streptococcus mutans UA140

53.666

95.518

0.513
  comGB Streptococcus mutans UA159

53.372

95.518

0.51
  comGB/cglB Streptococcus mitis SK321

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae D39

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae R6

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.448

93.838

0.473