Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LO002_RS04645 Genome accession   NZ_CP087321
Coordinates   966747..967865 (-) Length   372 a.a.
NCBI ID   WP_000347039.1    Uniprot ID   A0A9P2XLL8
Organism   Acinetobacter baumannii strain OC043     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 930410..996292 966747..967865 within 0


Gene organization within MGE regions


Location: 930410..996292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LO002_RS04465 - 930811..931371 (-) 561 WP_001048150.1 hypothetical protein -
  LO002_RS04470 - 931462..932322 (-) 861 WP_000838098.1 pirin family protein -
  LO002_RS04475 hemJ 932493..932945 (-) 453 WP_000339889.1 protoporphyrinogen oxidase HemJ -
  LO002_RS04480 - 932964..934193 (-) 1230 WP_000832710.1 beta-ketoacyl-ACP synthase II -
  LO002_RS04485 - 934490..935815 (+) 1326 WP_000160011.1 EcsC family protein -
  LO002_RS04490 rpsL 936016..936390 (+) 375 WP_000246374.1 30S ribosomal protein S12 -
  LO002_RS04495 rpsG 936550..937020 (+) 471 WP_001138055.1 30S ribosomal protein S7 -
  LO002_RS04500 fusA 937199..939337 (+) 2139 WP_000113824.1 elongation factor G -
  LO002_RS04505 tuf 939432..940622 (+) 1191 WP_001029610.1 elongation factor Tu -
  LO002_RS04510 - 940826..941707 (+) 882 WP_000992305.1 metal-dependent hydrolase -
  LO002_RS04515 - 941944..942819 (+) 876 WP_000992291.1 metal-dependent hydrolase -
  LO002_RS04520 rimI 942928..943383 (+) 456 WP_000621543.1 ribosomal protein S18-alanine N-acetyltransferase -
  LO002_RS04525 - 943428..944243 (-) 816 WP_000844343.1 arginyltransferase -
  LO002_RS04530 aat 944262..944993 (-) 732 WP_000854785.1 leucyl/phenylalanyl-tRNA--protein transferase -
  LO002_RS04535 trxB 945113..946060 (-) 948 WP_001276144.1 thioredoxin-disulfide reductase -
  LO002_RS04540 - 946330..949362 (+) 3033 WP_000127823.1 DNA translocase FtsK -
  LO002_RS04545 rhtC 949512..950132 (+) 621 WP_000959259.1 threonine export protein RhtC -
  LO002_RS04550 - 950188..950718 (-) 531 WP_031969348.1 tyrosyl-tRNA synthetase -
  LO002_RS04555 - 951053..951340 (-) 288 WP_001218560.1 PA4642 family protein -
  LO002_RS04560 minE 951424..951696 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  LO002_RS04565 minD 951699..952511 (-) 813 WP_001074362.1 septum site-determining protein MinD -
  LO002_RS04570 minC 952582..953304 (-) 723 WP_000763677.1 septum site-determining protein MinC -
  LO002_RS04575 - 953426..954472 (-) 1047 WP_001181639.1 hypothetical protein -
  LO002_RS04580 - 954489..955415 (-) 927 WP_000100965.1 acyltransferase -
  LO002_RS04585 - 955660..956313 (+) 654 WP_001202415.1 OmpA family protein -
  LO002_RS04590 rep 956507..958546 (+) 2040 WP_000093035.1 DNA helicase Rep -
  LO002_RS04595 dut 958571..959023 (+) 453 WP_000868152.1 dUTP diphosphatase -
  LO002_RS04600 - 959223..960641 (+) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  LO002_RS04605 argB 960656..961564 (+) 909 WP_001135419.1 acetylglutamate kinase -
  LO002_RS04610 - 961685..962467 (+) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  LO002_RS04615 - 962576..963424 (+) 849 WP_000336548.1 class II glutamine amidotransferase -
  LO002_RS04620 - 963677..964132 (+) 456 WP_000782976.1 bacteriohemerythrin -
  LO002_RS04625 - 964285..965352 (+) 1068 WP_002019572.1 hypothetical protein -
  LO002_RS04630 - 965352..965675 (+) 324 WP_000442587.1 RnfH family protein -
  LO002_RS04635 - 965730..966128 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  LO002_RS04640 fur 966240..966677 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  LO002_RS04645 pilU 966747..967865 (-) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LO002_RS04650 pilT 967893..968930 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  LO002_RS04655 - 969057..969749 (+) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  LO002_RS04660 - 969951..973547 (-) 3597 WP_000698785.1 AAA family ATPase -
  LO002_RS04665 - 973557..974825 (-) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  LO002_RS04670 - 974989..975462 (+) 474 WP_000974358.1 OsmC family protein -
  LO002_RS04675 - 975530..975907 (-) 378 WP_001059438.1 VOC family protein -
  LO002_RS04680 - 976287..976739 (+) 453 WP_000164153.1 ABZJ_00895 family protein -
  LO002_RS04685 - 976829..977734 (+) 906 WP_129194466.1 TIGR01777 family oxidoreductase -
  LO002_RS04690 - 977726..978841 (-) 1116 WP_000546696.1 FAD-binding oxidoreductase -
  LO002_RS04695 hemB 978867..979880 (-) 1014 WP_000222569.1 porphobilinogen synthase -
  LO002_RS04700 - 980023..980520 (+) 498 WP_002156418.1 thioesterase family protein -
  LO002_RS04705 - 980790..981941 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  LO002_RS04710 - 981948..983471 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  LO002_RS04715 ggt 983775..985760 (+) 1986 WP_001042913.1 gamma-glutamyltransferase -
  LO002_RS04725 - 986463..987692 (+) 1230 WP_001991065.1 tyrosine-type recombinase/integrase -
  LO002_RS04730 - 987840..988607 (+) 768 WP_000578618.1 hypothetical protein -
  LO002_RS04735 - 988817..989032 (+) 216 WP_000153154.1 hypothetical protein -
  LO002_RS04740 - 989035..989244 (+) 210 WP_001016479.1 helix-turn-helix domain-containing protein -
  LO002_RS04745 - 989241..989795 (+) 555 WP_001988245.1 BRO family protein -
  LO002_RS04750 - 989788..990156 (+) 369 WP_001046481.1 hypothetical protein -
  LO002_RS04755 - 990166..990471 (+) 306 WP_000787149.1 hypothetical protein -
  LO002_RS04760 - 990593..990871 (+) 279 WP_001064707.1 hypothetical protein -
  LO002_RS04765 - 990884..993349 (+) 2466 WP_001022397.1 DUF927 domain-containing protein -
  LO002_RS04770 - 993692..993808 (+) 117 WP_001992235.1 single-stranded DNA-binding protein -
  LO002_RS04775 - 994031..995473 (+) 1443 WP_000003535.1 hypothetical protein -
  LO002_RS04780 ssb 995455..995556 (+) 102 WP_168713084.1 single-stranded DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41933.13 Da        Isoelectric Point: 6.6944

>NTDB_id=628687 LO002_RS04645 WP_000347039.1 966747..967865(-) (pilU) [Acinetobacter baumannii strain OC043]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=628687 LO002_RS04645 WP_000347039.1 966747..967865(-) (pilU) [Acinetobacter baumannii strain OC043]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.565

98.925

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395