Detailed information NTG0628408

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LO009_RS04405
• Genome accession: NZ_CP087298
• Coordinates: 925840..926958 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347040.1
• Uniprot ID: A0A809JLP7
• Organism: Acinetobacter baumannii strain OC070
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 920840..931958

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LO009_RS04375	-	921669..922517 (+)	849	WP_000336551.1	class II glutamine amidotransferase	-
LO009_RS04380	-	922770..923225 (+)	456	WP_000782976.1	bacteriohemerythrin	-
LO009_RS04385	-	923378..924445 (+)	1068	WP_002084046.1	hypothetical protein	-
LO009_RS04390	-	924445..924768 (+)	324	WP_000442589.1	RnfH family protein	-
LO009_RS04395	-	924823..925221 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
LO009_RS04400	fur	925333..925770 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
LO009_RS04405	pilU	925840..926958 (-)	1119	WP_000347040.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LO009_RS04410	pilT	926986..928023 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
LO009_RS04415	-	928150..928842 (+)	693	WP_138149353.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41896.10 Da
• Isoelectric Point: 6.6406

>NTDB_id=628408 LO009_RS04405 WP_000347040.1 925840..926958(-) (pilU) [Acinetobacter baumannii strain OC070]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=628408 LO009_RS04405 WP_000347040.1 925840..926958(-) (pilU) [Acinetobacter baumannii strain OC070]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTTCGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAGGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACATTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A809JLP7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392