Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   N0B18_RS14325 Genome accession   NZ_CP103995
Coordinates   2640265..2641269 (-) Length   334 a.a.
NCBI ID   WP_003229718.1    Uniprot ID   O32055
Organism   Bacillus subtilis strain SRCM117109     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2635265..2646269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0B18_RS14295 (N0B18_14295) yrbG 2636239..2636895 (-) 657 WP_014480436.1 DUF421 domain-containing protein -
  N0B18_RS14300 (N0B18_14300) - 2637093..2637482 (+) 390 WP_014477528.1 TIGR04086 family membrane protein -
  N0B18_RS14305 (N0B18_14305) yajC 2637539..2637805 (-) 267 WP_004398708.1 preprotein translocase subunit YajC -
  N0B18_RS14310 (N0B18_14310) tgt 2637842..2638987 (-) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  N0B18_RS14315 (N0B18_14315) queA 2639014..2640042 (-) 1029 WP_014477530.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  N0B18_RS14320 (N0B18_14320) yrzS 2640072..2640272 (-) 201 WP_003222669.1 DUF2905 domain-containing protein -
  N0B18_RS14325 (N0B18_14325) ruvB 2640265..2641269 (-) 1005 WP_003229718.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  N0B18_RS14330 (N0B18_14330) ruvA 2641280..2641885 (-) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  N0B18_RS14335 (N0B18_14335) bofC 2642024..2642536 (-) 513 WP_014480438.1 sporulation cell-cell signaling protein BofC -
  N0B18_RS14340 (N0B18_14340) csbX 2642584..2643891 (-) 1308 WP_014480439.1 MFS transporter -
  N0B18_RS14345 (N0B18_14345) iolG 2643962..2644987 (-) 1026 WP_014480440.1 inositol 2-dehydrogenase -
  N0B18_RS14350 (N0B18_14350) yrzF 2645225..2645872 (+) 648 WP_014480441.1 phosphotransferase -
  N0B18_RS14355 (N0B18_14355) - 2645918..2646040 (-) 123 WP_003229707.1 hypothetical protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37467.78 Da        Isoelectric Point: 5.1631

>NTDB_id=626281 N0B18_RS14325 WP_003229718.1 2640265..2641269(-) (ruvB) [Bacillus subtilis strain SRCM117109]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=626281 N0B18_RS14325 WP_003229718.1 2640265..2641269(-) (ruvB) [Bacillus subtilis strain SRCM117109]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGCCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTAATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGTGATTTCGCCCAAGTGCTGGGTGACAGCAGGATC
ACTGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGATCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATATCAGCTACGATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518