Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LMH63_RS01440 Genome accession   NZ_CP086615
Coordinates   318783..319847 (-) Length   354 a.a.
NCBI ID   WP_369406039.1    Uniprot ID   -
Organism   Spiribacter halobius strain E85     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 313783..324847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH63_RS01415 (LMH63_01415) aroK 314214..314747 (-) 534 WP_109676203.1 shikimate kinase AroK -
  LMH63_RS01420 (LMH63_01420) pilQ 314850..316997 (-) 2148 WP_229332688.1 type IV pilus secretin PilQ family protein -
  LMH63_RS01425 (LMH63_01425) - 317050..317583 (-) 534 WP_109676200.1 pilus assembly protein PilP -
  LMH63_RS01430 (LMH63_01430) - 317580..318203 (-) 624 WP_109676199.1 type 4a pilus biogenesis protein PilO -
  LMH63_RS01435 (LMH63_01435) - 318203..318781 (-) 579 WP_109676198.1 PilN domain-containing protein -
  LMH63_RS01440 (LMH63_01440) pilM 318783..319847 (-) 1065 WP_369406039.1 pilus assembly protein PilM Machinery gene
  LMH63_RS01445 (LMH63_01445) - 320017..322524 (+) 2508 WP_109676196.1 penicillin-binding protein 1A -
  LMH63_RS01450 (LMH63_01450) - 322546..323154 (+) 609 WP_109676194.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38077.11 Da        Isoelectric Point: 4.4604

>NTDB_id=625701 LMH63_RS01440 WP_369406039.1 318783..319847(-) (pilM) [Spiribacter halobius strain E85]
MALFRKKPVPMLGIDVSSTSVKLIELRGHDQRFRVESFAVEPLAQNAVIEKNISNIEAVSEGIRAAVRRSQTKLKQAAVA
VPGSSAISRTLTLPAGLNDDELEAQVQLQADQYVPYPLEEVNLDFQIIGPAQRDPNEVEVLLVASRSENVDIRVDALEAA
GLKAQLVDVESYALEETFTRLIAPELPEASEGTVAVVDVGATMTTLTVFEAQQIVYTRDQVFGGRQLTDEIMRRYGLSYE
EAGRAKRQGGGLPDTYEEEVLAPFREAMSQQVGRSLQFFFGASQHNSVDHVVLAGGCAGVTGAAEAIADATGAPTTVANP
FARMSVASRVKPQALADDAPAMMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=625701 LMH63_RS01440 WP_369406039.1 318783..319847(-) (pilM) [Spiribacter halobius strain E85]
GTGGCCCTCTTCAGGAAGAAGCCCGTCCCGATGCTCGGCATCGACGTCAGCTCCACCTCGGTGAAGCTGATCGAGCTTCG
CGGGCATGACCAGCGTTTCCGGGTGGAGAGCTTCGCCGTGGAGCCCCTTGCCCAGAACGCGGTGATCGAAAAGAACATCA
GCAACATCGAGGCCGTGAGCGAGGGGATTCGCGCTGCCGTGCGCCGTTCCCAGACCAAGCTCAAGCAGGCCGCCGTCGCG
GTTCCGGGCTCTTCCGCCATCAGCCGCACGCTCACCCTGCCGGCCGGGCTCAACGACGACGAGCTCGAGGCCCAGGTTCA
GCTGCAGGCCGACCAGTATGTGCCCTATCCGCTGGAAGAGGTGAACCTCGACTTCCAGATCATCGGCCCCGCTCAGCGCG
ACCCCAACGAGGTCGAGGTGCTGCTGGTCGCCTCCCGCAGCGAGAACGTGGACATCCGTGTCGACGCGCTGGAGGCGGCA
GGCCTGAAGGCACAGCTGGTGGACGTCGAGAGCTATGCCCTCGAGGAGACCTTCACTCGGCTGATCGCCCCGGAGCTGCC
GGAGGCGTCCGAGGGCACCGTCGCGGTGGTGGATGTGGGCGCGACCATGACCACCCTCACCGTGTTCGAGGCCCAGCAGA
TCGTCTACACCCGGGACCAGGTCTTCGGCGGCCGCCAGCTCACCGACGAGATCATGCGCCGGTACGGGCTCTCGTACGAG
GAGGCCGGGCGCGCCAAGCGTCAGGGCGGCGGGCTGCCGGACACCTACGAGGAGGAGGTGCTGGCCCCGTTCCGCGAGGC
GATGAGCCAGCAGGTCGGGCGCTCGCTGCAGTTCTTCTTCGGCGCCAGCCAGCACAACAGCGTCGATCACGTCGTCCTTG
CGGGCGGCTGCGCCGGCGTCACCGGGGCCGCGGAGGCCATCGCCGATGCCACCGGGGCGCCCACCACCGTGGCCAACCCC
TTTGCCCGCATGTCCGTCGCCTCCCGGGTCAAGCCCCAGGCGCTGGCGGACGATGCTCCGGCCATGATGATCGCCTGCGG
GCTGGCGCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

49.435

100

0.494
  comM Acinetobacter nosocomialis M2

49.435

100

0.494
  comM Acinetobacter baylyi ADP1

45.763

100

0.458
  pilM Legionella pneumophila strain ERS1305867

41.127

100

0.412