Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LMF57_RS04685 Genome accession   NZ_CP086598
Coordinates   1027559..1028722 (+) Length   387 a.a.
NCBI ID   WP_049467229.1    Uniprot ID   -
Organism   Stenotrophomonas sp. SI-NJAU-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1022559..1033722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMF57_RS04655 (LMF57_04655) soxR 1022736..1023170 (+) 435 WP_032951587.1 redox-sensitive transcriptional activator SoxR -
  LMF57_RS04660 (LMF57_04660) - 1023208..1023810 (-) 603 WP_025874043.1 short chain dehydrogenase -
  LMF57_RS04665 (LMF57_04665) - 1023909..1024784 (+) 876 WP_117309690.1 LysR family transcriptional regulator -
  LMF57_RS04670 (LMF57_04670) proC 1024839..1025660 (-) 822 WP_117309691.1 pyrroline-5-carboxylate reductase -
  LMF57_RS04675 (LMF57_04675) - 1025677..1026354 (-) 678 WP_093817534.1 YggS family pyridoxal phosphate-dependent enzyme -
  LMF57_RS04680 (LMF57_04680) pilT 1026442..1027479 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  LMF57_RS04685 (LMF57_04685) pilU 1027559..1028722 (+) 1164 WP_049467229.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LMF57_RS04690 (LMF57_04690) - 1028793..1029446 (+) 654 WP_162887614.1 PIN domain-containing protein -
  LMF57_RS04695 (LMF57_04695) - 1029560..1030135 (+) 576 WP_140224771.1 hypothetical protein -
  LMF57_RS04700 (LMF57_04700) - 1030224..1031207 (+) 984 WP_117309694.1 aldo/keto reductase -
  LMF57_RS04705 (LMF57_04705) - 1031313..1032212 (-) 900 WP_033830519.1 LysR family transcriptional regulator -
  LMF57_RS04710 (LMF57_04710) - 1032423..1032635 (+) 213 WP_117309695.1 DUF4287 domain-containing protein -
  LMF57_RS04715 (LMF57_04715) - 1032908..1033615 (+) 708 WP_117309696.1 YitT family protein -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43182.62 Da        Isoelectric Point: 6.6616

>NTDB_id=625654 LMF57_RS04685 WP_049467229.1 1027559..1028722(+) (pilU) [Stenotrophomonas sp. SI-NJAU-1]
MPHWEPAVNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNQNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKELM
KESVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=625654 LMF57_RS04685 WP_049467229.1 1027559..1028722(+) (pilU) [Stenotrophomonas sp. SI-NJAU-1]
ATGCCCCATTGGGAGCCCGCCGTGAACACCACCGCGACCACCATCGATTTCACCTCGTTCCTCAAGTTGATGGCGCACCA
GCGCGCCTCGGACCTGTTCATCACTGCCGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATCTCGCCGATCACCCAGA
CGCCACTGACGCCGCAGCAAAGCCGCGACCTGGTGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAATTCGAAAAGACC
CACGAATGCAACTTCGCCATCGGCCTGTCCGGCGTTGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGG
CATGGTGCTGCGTCGCATCGAGACGCGCATCCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCGACCGGTACCGGTAAATCAACCTCGCTGGCAGCGATGATCGGTTAC
CGCAACCAGAATTCGACCGGGCACATCATCACCATCGAAGATCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCAT
CACCCAGCGCGAAGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGATGTGA
TCATGATCGGCGAGGTGCGTACCCGCGAGGGCATGGACCATGCCATCGCCTTTGCCGAAACCGGTCACCTGGTGCTGTGC
ACGCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGATCGCCGCAACCAGCTGTT
GATGGATCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGTTGATCCCTTCGCCGGATGGCCGCTCGCGCAAGGTGG
CGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAGCTGATG
AAGGAATCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAGATCAGCTACGA
AGACGCACTGCGCTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGATGCCCGCACCC
TGTCGCAGGGGTTGGATGGCGTGGAGATTTCAGAGGTCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

94.832

0.641
  pilU Acinetobacter baylyi ADP1

64.146

92.248

0.592
  pilU Vibrio cholerae strain A1552

53.371

91.99

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.817

91.731

0.393