Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   NYS59_RS27205 Genome accession   NZ_CP103789
Coordinates   5857389..5858012 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 2718     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5852389..5863012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYS59_RS27185 aroB 5852984..5854090 (-) 1107 WP_009314395.1 3-dehydroquinate synthase -
  NYS59_RS27190 aroK 5854140..5854658 (-) 519 WP_003095833.1 shikimate kinase AroK -
  NYS59_RS27195 pilQ 5854670..5856814 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  NYS59_RS27200 comL/pilP 5856868..5857392 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  NYS59_RS27205 pilO 5857389..5858012 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  NYS59_RS27210 pilN 5858009..5858605 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  NYS59_RS27215 pilM 5858605..5859669 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  NYS59_RS27220 - 5859854..5862325 (+) 2472 WP_031693142.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=625470 NYS59_RS27205 WP_003103268.1 5857389..5858012(-) (pilO) [Pseudomonas aeruginosa strain 2718]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=625470 NYS59_RS27205 WP_003103268.1 5857389..5858012(-) (pilO) [Pseudomonas aeruginosa strain 2718]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTTGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401