Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   M5T18_RS03835 Genome accession   NZ_CP103559
Coordinates   800481..801974 (+) Length   497 a.a.
NCBI ID   WP_000249317.1    Uniprot ID   -
Organism   Escherichia coli strain 3059     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 795481..806974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T18_RS03815 (M5T18_03815) pilD 796129..796938 (+) 810 WP_001377056.1 prepilin peptidase PppA Machinery gene
  M5T18_RS03820 (M5T18_03820) gspS2 797004..797414 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  M5T18_RS03825 (M5T18_03825) gspC 797432..798391 (+) 960 WP_000135091.1 type II secretion system protein GspC -
  M5T18_RS03830 (M5T18_03830) gspD 798421..800481 (+) 2061 WP_024210333.1 type II secretion system secretin GspD -
  M5T18_RS03835 (M5T18_03835) pilB/pilB1 800481..801974 (+) 1494 WP_000249317.1 type II secretion system ATPase GspE Machinery gene
  M5T18_RS03840 (M5T18_03840) gspF 801974..803197 (+) 1224 WP_001173433.1 type II secretion system inner membrane protein GspF -
  M5T18_RS03845 (M5T18_03845) gspG 803214..803669 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  M5T18_RS03850 (M5T18_03850) gspH 803673..804236 (+) 564 WP_001115152.1 type II secretion system minor pseudopilin GspH -
  M5T18_RS03855 (M5T18_03855) gspI 804233..804604 (+) 372 WP_000820108.1 type II secretion system minor pseudopilin GspI -
  M5T18_RS03860 (M5T18_03860) gspJ 804601..805206 (+) 606 WP_001254696.1 type II secretion system minor pseudopilin GspJ -
  M5T18_RS03865 (M5T18_03865) gspK 805203..806180 (+) 978 WP_000633199.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55222.37 Da        Isoelectric Point: 6.0390

>NTDB_id=623112 M5T18_RS03835 WP_000249317.1 800481..801974(+) (pilB/pilB1) [Escherichia coli strain 3059]
MVPVAQETSANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCTDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=623112 M5T18_RS03835 WP_000249317.1 800481..801974(+) (pilB/pilB1) [Escherichia coli strain 3059]
ATGGTGCCTGTAGCACAGGAAACCTCCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGCCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGTTGATGGAAGATCTCGGTTCCGCCGAGGACTTCTTTACCCTCGCAGAAGAGCTGCCAGAAACGGAAGA
TTTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATTAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTAGACGGAACGCTACATGAAATGCTGCGT
CCCGGGCGCAAACTAGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAACGTCTGGGGTTGAGTCAGCAACTGACC
GTGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCAGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTGCTTTCGACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTCGAGCCTTTCCTGC
TCTCTTCCAGCCTGACGGGCGTGATGGCGCAGCGGCTGGTCCGCACGCTGTGCACCGACTGCCGCCAGCCCGCGCCTGCC
ACTGATGAAGAAAAACGCCTGCTGGGGATTACCGACGCGCGCACCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTGCTGCGGGTCACGATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362